Ghai Ishan
Department of Life Sciences and Chemistry, Jacobs University Bremen, 28719 Bremen, Germany.
Membranes (Basel). 2024 Jul 20;14(7):161. doi: 10.3390/membranes14070161.
The alarming rise of antibiotic resistance in Gram-negative bacteria has emerged as a major global health challenge. A key factor contributing to this crisis is the low permeability of the bacterial outer membrane, which acts as a barrier that prevents antibiotics from entering the cell. Protein channels embedded in this outer membrane selectively regulate the influx of hydrophilic compounds, including antibiotics. To combat antibiotic resistance, understanding the molecular mechanisms governing antibiotic permeability through bacterial membrane channels is crucial. This knowledge is key towards elucidating their roles in studing antibiotic resistance. By compiling and analysing the flux data from multiple electrophysiological reversal potential experimental studies, which involves measuring zero-current potentials and the corresponding single-channel conductance, we can calculate the flux of charged antibiotics/compounds across different Gram-negative bacterial outer membrane channels. Through this comprehensive synthesis, this review aims to advance our understanding and stimulate discussions about the physicochemical factors influencing the flux of antibiotics through bacterial membrane protein channels, ultimately enhancing our knowledge in this area.
革兰氏阴性菌中抗生素耐药性的惊人上升已成为一项重大的全球健康挑战。导致这一危机的一个关键因素是细菌外膜的低通透性，它充当了阻止抗生素进入细胞的屏障。嵌入这种外膜的蛋白质通道选择性地调节亲水性化合物（包括抗生素）的流入。为了对抗抗生素耐药性，了解控制抗生素通过细菌膜通道的渗透的分子机制至关重要。这一知识对于阐明它们在研究抗生素耐药性中的作用至关重要。通过汇编和分析来自多个电生理逆转电位实验研究的通量数据，这些研究涉及测量零电流电位和相应的单通道电导，我们可以计算带电抗生素/化合物跨不同革兰氏阴性菌外膜通道的通量。通过这种全面的综合，本综述旨在增进我们的理解，并激发关于影响抗生素通过细菌膜蛋白通道通量的物理化学因素的讨论，最终增强我们在这一领域的知识。
