Yan Shengkun, Dong Rong
Agricultural Mechanization Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.
Metabolites. 2024 Jun 22;14(7):351. doi: 10.3390/metabo14070351.
In the conducted research, a murine model for ulcerative colitis (UC) was established utilizing dextran sodium sulfate (DSS) to investigate the therapeutic potential of dandelion root polysaccharide extracts on this disease. This study employed an analysis of gut microbiota composition and serum metabolomics to understand the biochemical effects of these polysaccharides. Sequencing of the 16S ribosomal DNA component indicated an increased presence of Bacteroides in the DSS-treated model group, contrasting with a significant enhancement in Faecalibaculum populations in mice treated with dandelion root polysaccharides (DPs). This shift suggests a pivotal role of DPs in elevating fecal N-butyric acid levels-a crucial factor in the maintenance of gut microbiota equilibrium. Through metabolomic profiling of serum, this research identified distinct metabolic changes across the control, DSS model, and DP treatment groups, highlighting four major differential metabolites: (2S)-2-amino-3-[[(2R)-2-butanoyloxy-3-propanoyloxypropoxy]-hydroxyphosphoryl]oxypropanoic acid; (1R,8S,9S)-3,4-dihydroxy-8-methoxy-11,11-dimethyl-5-propan-2-yl-16-oxatetracyclo [7.5.2.01,10.02,7]hexadeca-2,4,6-trien-15-one; Aspartylasparagine; and Nap-Phe-OH. These metabolites are implicated in mitigating oxidative stress, suggesting that DPs facilitate a protective mechanism for the intestinal lining through various biochemical pathways. Additionally, a notable correlation was established between the altered gut microbiota and the serum metabolomic profiles, underscoring the intricate interplay between these two biological systems in the context of UC. This study's outcomes illustrate that UC induces significant alterations in both gut microbiota and metabolic signatures, whereas dandelion root polysaccharides exhibit a profound ameliorative effect on these disruptions. This investigation underscores the therapeutic promise of dandelion root polysaccharides in the management of UC by modulating gut microbiota and metabolic pathways.
在进行的研究中，利用葡聚糖硫酸钠（DSS）建立了溃疡性结肠炎（UC）的小鼠模型，以研究蒲公英根多糖提取物对该疾病的治疗潜力。本研究采用肠道微生物群组成分析和血清代谢组学来了解这些多糖的生化作用。16S核糖体DNA成分测序表明，在DSS处理的模型组中拟杆菌的存在增加，而在用蒲公英根多糖（DPs）处理的小鼠中，粪杆菌种群显著增加。这种转变表明DPs在提高粪便中N-丁酸水平方面起着关键作用，而N-丁酸是维持肠道微生物群平衡的关键因素。通过血清代谢组学分析，本研究确定了对照组、DSS模型组和DP治疗组之间不同的代谢变化，突出了四种主要差异代谢物：（2S）-2-氨基-3-[[（2R）-2-丁酰氧基-3-丙酰氧基丙氧基]-羟基磷酰基]氧基丙酸；（1R，8S，9S）-3,4-二羟基-8-甲氧基-11,11-二甲基-5-丙-2-基-16-氧杂四环[7.5.2.01,10.02,7]十六碳-2,4,6-三烯-15-酮；天冬氨酰天冬酰胺；以及萘丙氨酸-OH。这些代谢物与减轻氧化应激有关，表明DPs通过各种生化途径促进肠道内膜的保护机制。此外，在肠道微生物群的改变与血清代谢组学谱之间建立了显著的相关性，强调了在UC背景下这两个生物系统之间的复杂相互作用。本研究结果表明，UC在肠道微生物群和代谢特征方面均引起了显著变化，而蒲公英根多糖对这些破坏具有显著的改善作用。这项研究强调了蒲公英根多糖通过调节肠道微生物群和代谢途径在UC治疗中的前景。
