不同解剖部位的抗生素耐受性患病率各异。

The Prevalence of Antibiotic Tolerance in Varies by Anatomical Site.

作者信息

Balduck Margaux, Strikker Akim, Gestels Zina, Abdellati Saïd, Van den Bossche Dorien, De Baetselier Irith, Kenyon Chris, Manoharan-Basil Sheeba Santhini

机构信息

STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium.

Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium.

出版信息

Pathogens. 2024 Jun 26;13(7):538. doi: 10.3390/pathogens13070538.

DOI:10.3390/pathogens13070538

PMID:39057765

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11279455/

Abstract

BACKGROUND

Tolerance enables bacteria to survive intermittent antibiotic exposure without an increase in antimicrobial susceptibility. In this study, we investigated the presence of tolerance to three antimicrobials, ceftriaxone, azithromycin and ciprofloxacin, in clinical isolates and the WHO (World Health Organization) reference panel of .

METHODS

We used the modified tolerance disk (TD test) to assess for tolerance to ceftriaxone, azithromycin and ciprofloxacin in 14 WHO reference strains and 62 clinical isolates-evenly divided between anorectal and urogenital infections. The isolates underwent a three-step incubation process wherein the isolates were exposed to an antibiotic disk for 20 h of incubation (Step I), followed by the replacement of the antibiotic disk with a nutrient disk for overnight incubation (Step II) and additional overnight incubation with extra nutrients (Step III).

RESULTS

A total of 4 of the 62 clinical anorectal isolates and none of the urogenital isolates exhibited tolerance to azithromycin ( = 0.033). Tolerance to ceftriaxone and ciprofloxacin was observed in eight and four isolates, respectively, with no difference between infection sites. Tolerance was also detected in 8 (K, M, N, O, P, U, V, W) out of the 14 WHO reference strains, with varying patterns of tolerance to ceftriaxone ( = 8), ciprofloxacin ( = 2) and azithromycin ( = 1).

CONCLUSIONS

This study identified ceftriaxone, azithromycin and ciprofloxacin tolerance in clinical and WHO reference isolates. Azithromycin tolerance was more common in anorectal than urogenital infections.

摘要

背景

耐受性使细菌能够在间歇性抗生素暴露下存活，而不会增加抗菌药物敏感性。在本研究中，我们调查了临床分离株和世界卫生组织（WHO）参考菌株中对三种抗菌药物（头孢曲松、阿奇霉素和环丙沙星）的耐受性情况。

方法

我们使用改良的耐受性纸片法（TD试验）评估14株WHO参考菌株和62株临床分离株（均匀分为肛门直肠感染和泌尿生殖系统感染）对头孢曲松、阿奇霉素和环丙沙星的耐受性。分离株经过三步培养过程，其中分离株先与抗生素纸片一起孵育20小时（第一步），然后用营养纸片替换抗生素纸片过夜孵育（第二步），再额外添加营养物过夜孵育（第三步）。

结果

62株临床肛门直肠分离株中有4株对阿奇霉素表现出耐受性，而泌尿生殖系统分离株均未表现出耐受性（P = 0.033）。分别在8株和4株分离株中观察到对头孢曲松和环丙沙星的耐受性，感染部位之间无差异。在14株WHO参考菌株中的8株（K、M、N、O、P、U、V、W）中也检测到耐受性，对头孢曲松（P = 8）、环丙沙星（P = 2）和阿奇霉素（P = 1）的耐受性模式各不相同。