Key Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, School of Pharmacy, Minzu University of China, Beijing 100081, China.
Laboratory of Toxicant Analysis, Academy of Military Medical Sciences, and State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China.
Toxins (Basel). 2024 Jul 11;16(7):312. doi: 10.3390/toxins16070312.
Abrin and ricin, both type II ribosome-inactivating proteins, are toxins of significant concern and are under international restriction by the Chemical Weapons Convention and the Biological and Toxin Weapons Convention. The development of a rapid and sensitive detection method for these toxins is of the utmost importance for the first emergency response. Emerging rapid detection techniques, such as surface-enhanced Raman spectroscopy (SERS) and lateral flow assay (LFA), have garnered attention due to their high sensitivity, good selectivity, ease of operation, low cost, and disposability. In this work, we generated stable and high-affinity nanotags, via an efficient freezing method, to serve as the capture module for SERS-LFA. We then constructed a sandwich-style lateral flow test strip using a pair of glycoproteins, asialofetuin and concanavalin A, as the core affinity recognition molecules, capable of trace measurement for both abrin and ricin. The limit of detection for abrin and ricin was 0.1 and 0.3 ng/mL, respectively. This method was applied to analyze eight spiked white powder samples, one juice sample, and three actual botanic samples, aligning well with cytotoxicity assay outcomes. It demonstrated good inter-batch and intra-batch reproducibility among the test strips, and the detection could be completed within 15 min, indicating the suitability of this SERS-LFA method for the on-site rapid detection of abrin and ricin toxins.
相思豆毒素和蓖麻毒素均属于 II 型核糖体失活蛋白，是具有重要意义的毒素，受到《化学武器公约》和《生物和毒素武器公约》的国际限制。开发一种快速、灵敏的检测这些毒素的方法对于第一时间的紧急响应至关重要。新兴的快速检测技术，如表面增强拉曼光谱（SERS）和侧向流分析（LFA），由于其高灵敏度、良好的选择性、易于操作、低成本和可一次性使用而受到关注。在这项工作中，我们通过一种高效的冷冻方法生成了稳定且高亲和力的纳米标签，用作 SERS-LFA 的捕获模块。然后，我们使用一对糖蛋白，即去唾液酸胎球蛋白和伴刀豆球蛋白 A，作为核心亲和识别分子，构建了一种夹心式侧向流测试条，能够痕量测量相思豆毒素和蓖麻毒素。相思豆毒素和蓖麻毒素的检测限分别为 0.1 和 0.3 ng/mL。该方法应用于分析 8 个掺假白色粉末样品、1 个果汁样品和 3 个实际植物样品，与细胞毒性测定结果吻合良好。它表明测试条之间具有良好的批间和批内重现性，检测可以在 15 分钟内完成，表明该 SERS-LFA 方法适用于相思豆毒素和蓖麻毒素的现场快速检测。
