University of Cambridge, Cancer Research UK Cambridge Institute, Li Ka Shing Centre, Robinson Way, CB2 0RE Cambridge, UK.
University of Cambridge, Cancer Research UK Cambridge Institute, Li Ka Shing Centre, Robinson Way, CB2 0RE Cambridge, UK.
STAR Protoc. 2024 Sep 20;5(3):103203. doi: 10.1016/j.xpro.2024.103203. Epub 2024 Jul 24.
Single-nuclei RNA sequencing (snRNA-seq) allows for obtaining gene expression profiles from frozen or hard-to-dissociate tissues at the single-nuclei level. Here, we describe a protocol to obtain snRNA-seq data of pancreatic tumors from orthotopically grafted organoid-derived mouse models. We provide details on the establishment of these mouse models, the isolation of single nuclei from pancreatic tumors, and the analysis of the snRNA-seq datasets. For complete details on the use and execution of this protocol, please refer to Mucciolo et al..
单细胞 RNA 测序（snRNA-seq）允许在单细胞水平上从冷冻或难以分离的组织中获得基因表达谱。在这里，我们描述了一种从原位移植的类器官衍生的小鼠模型中获取胰腺肿瘤 snRNA-seq 数据的方案。我们提供了关于这些小鼠模型的建立、从胰腺肿瘤中分离单细胞以及 snRNA-seq 数据集分析的详细信息。有关此方案使用和执行的完整详细信息，请参阅 Mucciolo 等人的研究。
