Pankey J W, Nickerson S C, Boddie R L, Hogan J S
J Dairy Sci. 1985 Oct;68(10):2684-93. doi: 10.3168/jds.S0022-0302(85)81153-X.
Experimental challenge procedures were used to study infectivity and virulence of Corynebacterium bovis. Challenge procedures using Staphylococcus aureus (Newbould 305) and Streptococcus agalactiae (McDonald 44) were used to study effects of Corynebacterium bovis infections on superinfection with major pathogens. Rate of infection under experimental challenge conditions was significantly greater with Corynebacterium bovis than previously observed for Staphylococcus aureus or Streptococcus agalactiae. Principal location of Corynebacterium bovis colonization appeared to the teat canal region, although the organism was isolated from 75% of the teat cisterns by puncture technique. Quarters with Corynebacterium bovis infections were more resistant to infection by Staphylococcus aureus than bacteriologically negative quarters. Quarters infected with Corynebacterium bacterium bovis were approximately 8.5-fold more susceptible to Streptococcus agalactiae infection than negative quarters. Somatic cell counts were doubled in negative quarters that developed Corynebacterium bovis infections; the geometric mean was 2.4 X 10(5).
采用实验性攻毒程序研究牛棒状杆菌的感染性和毒力。使用金黄色葡萄球菌（纽博尔德305株）和无乳链球菌（麦克唐纳44株）的攻毒程序来研究牛棒状杆菌感染对主要病原菌重复感染的影响。在实验性攻毒条件下，牛棒状杆菌的感染率显著高于先前观察到的金黄色葡萄球菌或无乳链球菌。牛棒状杆菌定植的主要部位似乎是乳头管区域，尽管通过穿刺技术从75%的乳头池分离到了该菌。感染牛棒状杆菌的乳腺区比细菌学检测为阴性的乳腺区对金黄色葡萄球菌感染更具抵抗力。感染牛棒状杆菌的乳腺区对无乳链球菌感染的易感性比阴性乳腺区高约8.5倍。发生牛棒状杆菌感染的阴性乳腺区体细胞计数增加了一倍；几何平均值为2.4×10⁵ 。
