Lv Xiaoci, Xie Yu, Li Heping
School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, No. 960, Wanjiali South Road, Tianxin District, Changsha, Hunan Province, 410114, China.
J Fluoresc. 2024 Jul 26. doi: 10.1007/s10895-024-03857-9.
Hydrogen sulfide (HS), as an important small molecule bioregulator, plays a key role in many physiological activities and signaling, and abnormal fluctuations in HS concentration can lead to a variety of diseases. Therefore, it is of great significance to develop a near-infrared fluorescence probe to visualize fluctuations in HS levels. This work is based on Sulfur-substituted dicyanomethylene-4 H-chromene (DCM), A novel NIR fluorescent probe (E) -3 - (2 - (4 - (dicyanomethylene) -6-methyl-4 H-Thiochromen-2-yl)vinyl-1-methylquinolin-1-ium (DMT) was synthesized successfully. Research has found that in weakly alkaline environments, the probe DMT reacts rapidly with HS (only 10 s), the fluorescence intensity at 684 nm is enhanced by about 60 fold, the detection limit is as low as 0.1623 µM, the Stokes shift is large (94 nm), and strong selectivity as well as anti-interference ability towards HS. This will provide a new method for the rapid detection and further application of HS.
硫化氢（HS）作为一种重要的小分子生物调节剂，在许多生理活动和信号传导中发挥着关键作用，HS浓度的异常波动会导致多种疾病。因此，开发一种近红外荧光探针来可视化HS水平的波动具有重要意义。本工作基于硫取代的二氰基亚甲基-4H-色烯（DCM），成功合成了一种新型近红外荧光探针（E）-3-（2-（4-（二氰基亚甲基）-6-甲基-4H-硫代色烯-2-基）乙烯基）-1-甲基喹啉-1-鎓（DMT）。研究发现，在弱碱性环境中，探针DMT与HS反应迅速（仅10秒），684nm处的荧光强度增强约60倍，检测限低至0.1623μM，斯托克斯位移大（94nm），对HS具有较强的选择性和抗干扰能力。这将为HS的快速检测和进一步应用提供一种新方法。
