Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250000, Shandong Province, China.
Jinan Municipal Center for Disease Control and Prevention, Jinan 250021, Shandong, China.
J Mater Chem B. 2022 Oct 12;10(39):7924-7954. doi: 10.1039/d2tb01210c.
HS, the smallest and simplest biological thiol in living systems, is the third member of the family of signaling mediators. HS participates in the regulation of a series of complex physiological and pathological functions in the body, making it a critical fulcrum that balances health and disease in human physiology. Small-molecule fluorescent probes have been proven to possess the unique advantages of high temporal and spatial resolution, good biocompatibility and high sensitivity, and thus their use is a powerful approach for monitoring the level and dynamics of HS in living cells and organisms and better understanding its basic cellular functions. The field of small-molecule fluorescent probes for monitoring the complex biological activities of HS has been thriving in recent years. Herein, we systematically summarize the latest developments in the field of fluorescent probes for the detection of HS, illustrate their biological applications according to the classification of target-responsive sites, and emphasize the development direction and challenges of HS-responsive fluorescent probes, hoping to give implications of researchers on fluorescent probes for future research.
HS 是生物体系中最小、最简单的生物硫醇，是信号转导介质家族的第三个成员。HS 参与体内一系列复杂的生理和病理功能的调节，是人体生理学中平衡健康与疾病的关键支点。小分子荧光探针具有时间和空间分辨率高、生物相容性好、灵敏度高等独特优势，因此，它们的使用是监测活细胞和生物体中 HS 水平和动态以及更好地理解其基本细胞功能的有力手段。近年来，监测 HS 复杂生物活性的小分子荧光探针领域蓬勃发展。本文系统总结了 HS 荧光探针检测领域的最新进展，根据靶标响应位点的分类说明了它们的生物学应用，并强调了 HS 响应荧光探针的发展方向和挑战，希望为未来研究的荧光探针研究人员提供启示。
