Kim Seong-Yeop, Park Seong-Yong, Lee Jung-Hwa, Kim Nayeong, Oh Ha-Na, Yoo So-Young, Lee Dae-Sung, Lee Je-Chul
Department of Microbiology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
Medi Bio Lab Co., Ltd., Seoul 08389, Republic of Korea.
Antibiotics (Basel). 2024 Jul 1;13(7):612. doi: 10.3390/antibiotics13070612.
α-mangostin (α-MG) demonstrates antibacterial activity against species. Therefore, this study aimed to explore the antibacterial activity of α-MG-rich mangosteen pericarp extract (MPE)-loaded liposomes against isolates from companion animal skin diseases in vitro and evaluated their therapeutic potential in a murine model of superficial skin infection caused by . α-MG-rich extract was purified from mangosteen pericarp and then complexed with γ-cyclodextrin (γ-CD), forming the inclusion complexes. Nanoliposomes containing MPE and γ-CD complexes were prepared by adding lecithin and casein. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of MPE-loaded liposomes were determined using agar dilution and broth microdilution methods. The therapeutic potential of MPE-loaded liposomes was evaluated in vivo on tape-stripped skin lesions infected with . Purified MPE and MPE-loaded liposomes contained 402.43 mg/g and 18.18 mg/g α-MG, respectively. MPE-loaded liposomes showed antibacterial activity against clinical isolates in vitro but did not show antibacterial activity against Gram-negative bacterial isolates. MPE-loaded liposomes demonstrated consistent MICs and MBCs against isolates. These liposomes significantly reduced bacterial numbers and lesional sizes in a superficial skin infection model. Moreover, they reconstructed the epidermal barrier in skin lesions. The therapeutic concentrations of MPE-loaded liposomes did not induce cytotoxicity in canine progenitor epidermal keratinocyte cells. In conclusion, MPE-loaded liposomes hold promise for the development of a prospective topical formulation to treat superficial pyoderma in companion animals.
α-山竹黄酮（α-MG）对多种菌种具有抗菌活性。因此，本研究旨在探讨富含α-MG的山竹果皮提取物（MPE）脂质体对伴侣动物皮肤疾病分离菌株的体外抗菌活性，并在由[具体病菌名称未给出]引起的浅表皮肤感染小鼠模型中评估其治疗潜力。从山竹果皮中纯化出富含α-MG的提取物，然后与γ-环糊精（γ-CD）复合，形成包合物。通过添加卵磷脂和酪蛋白制备含有MPE和γ-CD复合物的纳米脂质体。采用琼脂稀释法和肉汤微量稀释法测定负载MPE的脂质体的最低抑菌浓度（MICs）和最低杀菌浓度（MBCs）。在感染了[具体病菌名称未给出]的胶带剥离皮肤损伤上，对负载MPE的脂质体的治疗潜力进行体内评估。纯化的MPE和负载MPE的脂质体分别含有402.43 mg/g和18.18 mg/g的α-MG。负载MPE的脂质体在体外对临床[具体病菌名称未给出]分离菌株显示出抗菌活性，但对革兰氏阴性菌分离菌株未显示出抗菌活性。负载MPE的脂质体对[具体病菌名称未给出]分离菌株表现出一致的MICs和MBCs。这些脂质体在浅表皮肤感染模型中显著减少了细菌数量和损伤大小。此外，它们重建了皮肤损伤处的表皮屏障。负载MPE的脂质体的治疗浓度在犬祖表皮角质形成细胞中未诱导细胞毒性。总之，负载MPE的脂质体有望开发出一种用于治疗伴侣动物浅表脓疱病的前瞻性局部制剂。
