Arif Waseema, Thangaraj Gowdham, Srinivasan Pushpa, Srirama Srikanth, Devaraju Panneer
Unit of One Health, ICMR-Vector Control Research Centre, Puducherry 605006, India.
Public Health Entomology, ICMR-Vector Control Research Centre, Puducherry 605006, India.
Antibiotics (Basel). 2024 Jul 2;13(7):618. doi: 10.3390/antibiotics13070618.
The emergence of antimicrobial resistance (AMR) in pathogens and their spillover into the environment have become a global public health menace. Insects can acquire these pathogens from the environment and would serve as mechanical and biological vectors. The current study assessed the ability of mosquitoes to acquire methicillin-resistant (MRSA) through the exposure of the mosquitoes to the pathogen via rearing water, blood feed, or a feeding membrane under laboratory conditions. In addition, mosquito immatures collected from their habitat in the vicinity of hospitals, veterinary dispensaries, and butcher shops at 15 study sites in Puducherry were screened for MRSA infection. All samples were subjected to the culture-based isolation of from the surface and homogenate. The presence of the -specific gene and the genes that confer resistance to methicillin ( and ) were screened using PCR tests. MRSA was not evident either on the external surface or in the homogenate of the mosquitoes emerging from the larvae reared in the MRSA-spiked water or those fed with MRSA through blood or smeared membranes. Furthermore, the presence of MRSA was not evident in any of the field-caught mosquitoes. Hence, we conclude that mosquitoes are impervious to MRSA colonization.
病原体中抗菌药物耐药性（AMR）的出现及其向环境中的溢出已成为全球公共卫生威胁。昆虫可从环境中获取这些病原体，并充当机械和生物传播媒介。当前研究评估了蚊子在实验室条件下通过饲养用水、血液喂食或喂食膜接触病原体来获取耐甲氧西林金黄色葡萄球菌（MRSA）的能力。此外，在本地治里15个研究地点的医院、兽医诊所和肉店附近的蚊子栖息地收集的蚊幼虫，被筛查是否感染MRSA。所有样本均进行基于培养的表面和匀浆分离。使用PCR检测筛选特定基因以及赋予对甲氧西林耐药性的基因（mecA和mecC）的存在情况。在添加了MRSA的水中饲养的幼虫或通过血液或涂抹膜喂食了MRSA的幼虫所羽化出的蚊子的外表面或匀浆中，均未发现MRSA。此外，在任何野外捕获的蚊子中均未发现MRSA。因此，我们得出结论，蚊子对MRSA定植具有抗性。
