Devanathan Nivedha, Mukhopadhyay Hirak Kumar, Sihag Krishan Kumar, Terence Nathan A, Chakkaravarthi Aravindasamy, Srinivasan Lakshmy, Srinivas Mouttou Vivek, Vasu Jayalakshmi, Shanmugam Venkatesa Perumal, Rahi Manju, Devaraju Panneer
Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Kurumbapet, Puducherry 605009, India.
Unit of One Health, ICMR- Vector Control Research Centre (ICMR-VCRC), Indira Nagar, Puducherry 605006, India.
One Health. 2024 May 17;18:100759. doi: 10.1016/j.onehlt.2024.100759. eCollection 2024 Jun.
Antimicrobial resistance (AMR) is a global public health concern and needs to be monitored for control. In this study, synanthropic rodents trapped from humans and animal habitats in Puducherry, India, were screened as sentinels for bacterial pathogens of public health importance and antimicrobial resistance spillover. From the trapped rodents and shrews ( = 100) pathogens viz., and were isolated from oropharyngeal and rectal swabs on Mannitol salt, Mac Conkey and Xylose lysine deoxycholate media respectively. The AMR genes in these isolates were screened by PCR. A total of 76, and 19, non were isolated. was isolated in 89 samples and among the ( = 59), 16, were and 29, were A total of 46 MRSA isolates with ( = 40) ( = 6) were detected. Also, 36.84% and 5.3% non isolates were tested to have and genes. AMR genes encoding ESBL [bla in 21, bla in 45 and bla in 11] was tested positive in 77 isolates Among, isolates 44/45 were screened to have AMR genes [ in 13, in 20 and in 11]. Antibiotic sensitivity test confirmed the antimicrobial resistance. Isolation of pathogens of public health importance and demonstration of genetic elements conferring antimicrobial resistance in the synanthropic rodents confirms that they act as reservoirs and appropriate sentinels to monitor AMR spillover in the environment.
抗菌药物耐药性（AMR）是一个全球公共卫生问题，需要对其进行监测以加以控制。在本研究中，从印度本地治里的人类和动物栖息地捕获的伴人鼠类被作为具有公共卫生重要性的细菌病原体和抗菌药物耐药性溢出的哨兵进行筛查。从捕获的鼠类和鼩鼱（n = 100）中，分别在甘露醇盐培养基、麦康凯培养基和木糖赖氨酸脱氧胆酸盐培养基上从口咽和直肠拭子中分离出病原体，即金黄色葡萄球菌和大肠杆菌。通过聚合酶链反应（PCR）筛查这些分离株中的AMR基因。总共分离出76株金黄色葡萄球菌和19株大肠杆菌，无其他菌株。在89个样本中分离出金黄色葡萄球菌，在金黄色葡萄球菌分离株（n = 59）中，16株对甲氧西林敏感，29株对甲氧西林耐药。共检测到46株耐甲氧西林金黄色葡萄球菌（MRSA）分离株，其中40株携带mecA基因，6株携带mecC基因。此外，36.84%的甲氧西林敏感金黄色葡萄球菌分离株和5.3%的大肠杆菌分离株被检测到具有blaCTX-M和blaTEM基因。编码超广谱β-内酰胺酶（ESBL）的AMR基因[blaCTX-M-15在21株中、blaCTX-M-1在45株中、blaTEM在11株中]在77株大肠杆菌分离株中检测呈阳性。在金黄色葡萄球菌分离株中，44/45株被筛查出具有AMR基因[blaTEM在13株中、blaCTX-M在20株中、blaSHV在11株中]。抗生素敏感性试验证实了抗菌药物耐药性。在伴人鼠类中分离出具有公共卫生重要性的病原体并证明了赋予抗菌药物耐药性的遗传元件，这证实它们可作为监测环境中AMR溢出的储存宿主和合适的哨兵。
