Guzman Kevin Andres, Hidalgo Arsenio, Pazos Alvaro Jairo
Grupo Salud Pública, Centro de Estudios en Salud de la Universidad de Nariño (CESUN), Universidad de Nariño, Pasto 520001, Colombia.
Departamento de Matemáticas y Estadística, Facultad de Ciencias Exactas y Naturales, Universidad de Nariño, Pasto 520001, Colombia.
Antibiotics (Basel). 2024 Jul 12;13(7):643. doi: 10.3390/antibiotics13070643.
The eradication of is a valid strategy for preventing gastric cancer; however, the therapeutic failure of first-line treatments in Colombia is associated with high resistance to metronidazole and amoxicillin. This study explored alternative antibiotics and analyzed point mutations in resistance genes to furazolidone and rifampicin in order to include them in rescue therapy regimens. A total of 239 complete genomes of Colombian strains were compared to that of the ATCC 26695 strain to identify mutations in the and genes for rifampicin and furazolidinone resistance, respectively. While rifampicin resistance mutations were not found, only 0.84% of the isolates showed the gene, suggesting that is sensitive to these antibiotics. A phylogenomic analysis of revealed an independent lineage in Colombia (hspColombia). The absence of point mutations in the gene, together with the scarce mutations identified in the gene of , suggest that the hspColombia isolates are sensitive to rifampicin and furazolidone, which could be key to including these antibiotics in the rescue therapies against .
根除幽门螺杆菌是预防胃癌的有效策略；然而，哥伦比亚一线治疗的治疗失败与对甲硝唑和阿莫西林的高耐药性有关。本研究探索了替代抗生素，并分析了耐药基因中对呋喃唑酮和利福平的点突变，以便将它们纳入挽救治疗方案。将总共239个哥伦比亚菌株的完整基因组与ATCC 26695菌株的基因组进行比较，以分别鉴定利福平和呋喃唑酮耐药性的rpoB和frxA基因中的突变。虽然未发现利福平耐药突变，但仅0.84%的分离株显示frxA基因，表明幽门螺杆菌对这些抗生素敏感。对幽门螺杆菌的系统基因组分析揭示了哥伦比亚的一个独立谱系（hspColombia）。rpoB基因中没有点突变，以及幽门螺杆菌frxA基因中鉴定出的少量突变，表明hspColombia分离株对利福平和呋喃唑酮敏感,这可能是将这些抗生素纳入抗幽门螺杆菌挽救治疗的关键。
