Su Zhaoliang, Xu Huaxi, Zhang Chiyu, Shao Shihe, Li Liangju, Wang Hua, Wang Huifang, Qiu Gufeng
Department of Microbiology and Immunology, Institute of Clinical Laboratory Science, Jiangsu University, Zhenjiang, China.
Croat Med J. 2006 Jun;47(3):410-5.
To determine the rate of furazolidone resistance of Helicobacter pylori (H. pylori) isolated from gastric biopsy specimens and to explore the relationship between genetic mutations in porD and oorD genes of H. pylori and its resistance to the antibiotic.
Gastric biopsy was performed in 83 adult patients aged 31-77 years with gastric complaints. H. pylori was isolated from biopsy specimens of 46 patients. E-test and 2-fold agar dilution method were used to determine the rate of H. pylori resistance to furazolidone. The genes porD and oorD from susceptible and resistant isolates were amplified by polymerase chain reaction (PCR), and their PCR products were sequenced.
Resistance to furazolidone was found in 8.7% of H. pylori isolates and 6 mutations were detected in porD and oorD genes of the resistant isolates. Three mutations--G353A, A356G, and C357T--occurred in porD and the other mutations--A041G, A122G, C349A(G)--occurred in oorD genes.
Changes in 6 amino acids may be associated with the resistance of H. pylori to furazolidone.
确定从胃活检标本中分离出的幽门螺杆菌（H. pylori）对呋喃唑酮的耐药率，并探讨幽门螺杆菌porD和oorD基因的基因突变与其对抗生素耐药性之间的关系。
对83例年龄在31至77岁有胃部不适症状的成年患者进行胃活检。从46例患者的活检标本中分离出幽门螺杆菌。采用E-test法和2倍琼脂稀释法测定幽门螺杆菌对呋喃唑酮的耐药率。通过聚合酶链反应（PCR）扩增敏感和耐药菌株的porD和oorD基因，并对其PCR产物进行测序。
在8.7%的幽门螺杆菌分离株中发现对呋喃唑酮耐药，在耐药菌株的porD和oorD基因中检测到6个突变。3个突变——G353A、A356G和C357T——发生在porD基因中，其他突变——A041G、A122G、C349A（G）——发生在oorD基因中。
6个氨基酸的变化可能与幽门螺杆菌对呋喃唑酮的耐药性有关。
