Mutavdzin Krneta Slavica, Gopcevic Kristina, Stankovic Sanja, Jakovljevic Uzelac Jovana, Todorovic Dušan, Labudovic Borovic Milica, Rakocevic Jelena, Djuric Dragan
Institute of Medical Physiology "Richard Burian", Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia.
Institute of Chemistry in Medicine "Prof. Dr. Petar Matavulj", Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia.
Diagnostics (Basel). 2024 Jul 12;14(14):1507. doi: 10.3390/diagnostics14141507.
The aims of this study were to examine the effects of pyridoxine administration on the activities of cardiac antioxidant stress enzymes superoxide dismutase (SOD) and catalase (CAT) and enzyme indicators of cardiometabolic status, lactate and malate dehydrogenase (LDH, MDH), as well as LDH and MDH isoforms' distribution in the cardiac tissue of healthy and diabetic Wistar male rats. Experimental animals were divided into five groups: C1-control (0.9% sodium chloride-NaCl-1 mL/kg, intraperitoneally (i.p.), 1 day); C2-second control (0.9% NaCl 1 mL/kg, i.p., 28 days); DM-diabetes mellitus (streptozotocin 100 mg/kg in 0.9% NaCl, i.p., 1 day); P-pyridoxine (7 mg/kg, i.p., 28 days); and DM + P-diabetes mellitus and pyridoxine (streptozotocin 100 mg/kg, i.p., 1 day and pyridoxine 7 mg/kg, i.p., 28 days). Pyridoxine treatment reduced CAT and MDH activity in diabetic rats. In diabetic rats, the administration of pyridoxine increased LDH1 and decreased LDH4 isoform activities, as well as decreased peroxisomal MDH and increased mitochondrial MDH activities. Our findings highlight the positive effects of pyridoxine administration on the complex interplay between oxidative stress, antioxidant enzymes, and metabolic changes in diabetic cardiomyopathy.
本研究的目的是检测吡哆醇给药对健康和糖尿病雄性Wistar大鼠心脏抗氧化应激酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及心脏代谢状态酶指标乳酸和苹果酸脱氢酶（LDH、MDH）的影响，以及LDH和MDH同工酶在心脏组织中的分布。实验动物分为五组：C1-对照组（0.9%氯化钠-NaCl-1 mL/kg，腹腔注射（i.p.），1天）；C2-第二对照组（0.9% NaCl 1 mL/kg，i.p.，28天）；DM-糖尿病组（链脲佐菌素100 mg/kg溶于0.9% NaCl中，i.p.，1天）；P-吡哆醇组（7 mg/kg，i.p.，28天）；以及DM + P-糖尿病合并吡哆醇组（链脲佐菌素100 mg/kg，i.p.，1天，吡哆醇7 mg/kg，i.p.，28天）。吡哆醇治疗降低了糖尿病大鼠的CAT和MDH活性。在糖尿病大鼠中，吡哆醇给药增加了LDH1活性，降低了LDH4同工酶活性，同时降低了过氧化物酶体MDH活性，增加了线粒体MDH活性。我们的研究结果突出了吡哆醇给药对糖尿病性心肌病氧化应激、抗氧化酶和代谢变化之间复杂相互作用的积极影响。
