载聚多巴胺修饰富勒烯纳米复合材料的原位形成 ROS 清除杂化水凝胶促进皮肤伤口愈合。

In situ forming ROS-scavenging hybrid hydrogel loaded with polydopamine-modified fullerene nanocomposites for promoting skin wound healing.

机构信息

Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, PR China.

Department of Bone and Joint Surgery, Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandong Middle Road, Shanghai, 200001, PR China.

出版信息

J Nanobiotechnology. 2023 Apr 13;21(1):129. doi: 10.1186/s12951-023-01879-2.

BACKGROUND

Excessive oxidative stress at the wound sites always leads to a prolonged healing and even causes chronic inflammatory wounds. Therefore, antioxidative dressings with multiple features are desired to improve wound healing performance. Herein, we fabricated a ROS-scavenging hybrid hydrogel by incorporating mussel-inspired fullerene nanocomposites (C60@PDA) into gelatin methacryloyl (GelMA) hydrogel.

RESULTS

The developed C60@PDA/GelMA hydrogel showed a sustainable free radical scavenging ability, and eliminated ROS to protect cells against external oxidative stress damage. Besides, the hydrogel presented favorable cytocompatibility, hemocompatibility, and antibacterial ability in vitro. Furthermore, in a mouse full-thickness wound defect model, the in situ forming hybrid hydrogel accelerated wound closure by 38.5% and 42.9% on day 3 and day 7 over the control. Histological results demonstrated that hybrid hydrogels effectively enhanced wound healing on re-epithelialization, collagen deposition and angiogenesis.

CONCLUSION

Collectively, the C60@PDA/GelMA hydrogel could be a promising dressing for promoting cutaneous wound repair.

背景

伤口部位的过度氧化应激总是导致愈合时间延长，甚至导致慢性炎症性伤口。因此，需要具有多种功能的抗氧化敷料来改善伤口愈合性能。本文通过将贻贝启发的富勒烯纳米复合材料（C60@PDA）掺入明胶甲基丙烯酰（GelMA）水凝胶中，制备了一种 ROS 清除杂化水凝胶。

结果

所开发的 C60@PDA/GelMA 水凝胶具有可持续的自由基清除能力，可以消除 ROS，保护细胞免受外部氧化应激损伤。此外，该水凝胶在体外表现出良好的细胞相容性、血液相容性和抗菌能力。此外，在小鼠全层伤口缺陷模型中，原位形成的杂化水凝胶在第 3 天和第 7 天分别比对照组加快了 38.5%和 42.9%的伤口闭合。组织学结果表明，杂化水凝胶在再上皮化、胶原沉积和血管生成方面有效促进了伤口愈合。