Vinh Le Ba, Shin Seung Hyuck, Han Yoo Kyong, Kim Young Jun, Cuong Nguyen Cao, Oh Soohwan, Lee Ki Yong
College of Pharmacy, Korea University, Sejong 30019, Republic of Korea.
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, Hanoi 11355, Vietnam.
Antioxidants (Basel). 2024 Jun 25;13(7):767. doi: 10.3390/antiox13070767.
Interleukin (IL)-33, a member of the IL-1 cytokine family, plays a vital role in immune system regulation and inflammation, with oxidative stress being implicated in its expression. During the search for compounds from natural sources with potential as therapeutic agents for allergic diseases via IL-33 signal modulation, we discovered significant IL-33 inhibitory activity in the methanol extract of (sword bean) pods. Through chromatographic separation and liquid chromatography-mass spectrometry, we isolated 11 compounds (-) from the methanol extract. Furthermore, we assessed the inhibitory effects of these substances on IL-33/ST2 signaling in processes related to inflammatory and autoimmune diseases using an enzyme-linked immunosorbent assay. Among them, compounds , , and exhibited substantial IL-33 inhibitory efficacy, with values reaching 78%, 86%, and 79% at 100 µM, respectively. Remarkably, compounds , , and demonstrated significant and dose-dependent inhibition of IL-33 signaling at concentrations of 10, 50, and 100 µM. Computational molecular docking and dynamic simulations further elucidated the underlying mechanisms. These findings have promising pharmacological implications for allergy prevention and treatment associated with flavonoid glycosides derived from
白细胞介素（IL）-33是IL-1细胞因子家族的成员，在免疫系统调节和炎症中起重要作用，氧化应激与其表达有关。在通过IL-33信号调节从天然来源寻找具有作为过敏性疾病治疗剂潜力的化合物的过程中，我们在刀豆荚的甲醇提取物中发现了显著的IL-33抑制活性。通过色谱分离和液相色谱-质谱联用，我们从甲醇提取物中分离出11种化合物（-）。此外，我们使用酶联免疫吸附测定法评估了这些物质在与炎症和自身免疫性疾病相关的过程中对IL-33/ST2信号传导的抑制作用。其中，化合物、和表现出显著的IL-33抑制功效，在100μM时抑制率分别达到78%、86%和79%。值得注意的是，化合物、和在10、50和100μM浓度下对IL-33信号传导表现出显著的剂量依赖性抑制。计算分子对接和动态模拟进一步阐明了潜在机制。这些发现对于与源自的黄酮糖苷相关的过敏预防和治疗具有有前景的药理学意义
