Turk Ayman, Lee Solip, Yeon Sang Won, Ryu Se Hwan, Han Yoo Kyong, Kim Young Jun, Ko Sung Min, Kim Beom Seok, Hwang Bang Yeon, Lee Ki Yong, Lee Mi Kyeong
College of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of Korea.
College of Pharmacy, Korea University, Sejong 47236, Republic of Korea.
Antioxidants (Basel). 2023 Jun 12;12(6):1260. doi: 10.3390/antiox12061260.
Cordycepin, also known as 3'-deoxyadenosine, is a major active ingredient of with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were supplemented with cultivation substrates of to increase the cordycepin content. cultivated on brown rice supplemented with Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigantis Radix exhibited increased cordycepin content compared to a brown rice control. Among them, the addition of 25% Mori Folium increased the cordycepin content up to 4 times. Adenosine deaminase (ADA) modulates the deamination of adenosine and deoxyadenosine, and the inhibitors have therapeutic potential with anti-proliferative and anti-inflammatory properties. As ADA is also known to be involved in converting cordycepin to 3'-deoxyinosine, the inhibitory activity of medicinal plants on ADA was measured by spectrophotometric analysis using cordycepin as a substrate. As expected, Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigas Radix strongly inhibited ADA activity. Molecular docking analysis also showed the correlation between ADA and the major components of these medicinal plants. Conclusively, our research suggests a new strategy of using medicinal plants to enhance cordycepin production in .
虫草素，又称3'-脱氧腺苷，是冬虫夏草的一种主要活性成分，具有多种药理作用。由于其供应有限，人们进行了许多尝试来提高虫草素含量。作为本研究的一部分，向8种药用植物添加冬虫夏草的栽培基质以增加虫草素含量。与糙米对照相比，在添加了桑叶、姜黄、三白草和当归的糙米上培养的冬虫夏草表现出虫草素含量增加。其中，添加25%的桑叶可使虫草素含量提高至4倍。腺苷脱氨酶（ADA）调节腺苷和脱氧腺苷的脱氨作用，其抑制剂具有抗增殖和抗炎特性的治疗潜力。由于已知ADA也参与将虫草素转化为3'-脱氧肌苷，因此以虫草素为底物，通过分光光度分析测定药用植物对ADA的抑制活性。正如预期的那样，桑叶、姜黄、三白草和当归强烈抑制ADA活性。分子对接分析也显示了ADA与这些药用植物主要成分之间的相关性。总之，我们的研究提出了一种利用药用植物提高冬虫夏草中虫草素产量的新策略。
