Tang Kuo-Tung, Chen Yu-Sin, Lee Mey-Fann, Chen Tzu-Ting, Lai Chien-Chen, Lin Chi-Chien, Chen Yi-Hsing
Division of Allergy, Immunology and Rheumatology, Taichung Veterans General Hospital, Taichung 407, Taiwan.
School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
Biomedicines. 2024 Jun 26;12(7):1419. doi: 10.3390/biomedicines12071419.
Volatile organic compounds (VOC) are major indoor air pollutants. Previous studies reported an association between VOC exposure and allergic diseases. Here, we aimed to explore the relationship between VOC exposure and atopic dermatitis (AD) in adults.
We prospectively enrolled 31 adult AD patients and 11 healthy subjects as controls. Urine metabolite levels of VOCs, including 1.3-butadiene, acrylamide, benzene, toluene, and xylene, were all determined with liquid chromatography-mass spectrometry. The relationship between AD and log-transformed urine levels of VOC metabolites were examined using a multivariate linear regression model adjusted for age and sex. We also treated mouse bone marrow-derived cells (BMMCs) with 1,3-butadiene and toluene and measured the release of β-hexosaminidase.
Our results demonstrated that creatinine-corrected urine levels of N-Acetyl-S- (3,4-dihydroxybutyl)-L-cysteine (DHBMA), N-Acetyl-S-(2-carbamoyl-2-hydroxyethyl)-L-cysteine (GAMA), and N-Acetyl-S-(benzyl)-L-cysteine (BMA) were all elevated in AD patients compared with controls. In a multivariate linear regression model, creatinine-corrected urine levels of BMA (a toluene metabolite) and DHBMA (a 1,3-butadiene metabolite) appeared elevated in AD patients, although statistical significance was not reached after correction for multiple comparisons. In addition, 1,3-butadiene and toluene could stimulate BMMCs to degranulate as much as compound 48/80.
Some VOCs, such as 1,3-butadiene and toluene, might be associated with AD pathogenesis in adults.
挥发性有机化合物（VOC）是主要的室内空气污染物。先前的研究报道了VOC暴露与过敏性疾病之间的关联。在此，我们旨在探讨成人VOC暴露与特应性皮炎（AD）之间的关系。
我们前瞻性地招募了31名成年AD患者和11名健康受试者作为对照。使用液相色谱 - 质谱法测定尿液中VOCs的代谢物水平，包括1,3 - 丁二烯、丙烯酰胺、苯、甲苯和二甲苯。使用针对年龄和性别的多元线性回归模型检查AD与VOC代谢物对数转换后的尿液水平之间的关系。我们还用1,3 - 丁二烯和甲苯处理小鼠骨髓来源的细胞（BMMCs），并测量β - 己糖胺酶的释放。
我们的结果表明，与对照组相比，AD患者中N - 乙酰 - S - （3,4 - 二羟基丁基）- L - 半胱氨酸（DHBMA）、N - 乙酰 - S - （2 - 氨基甲酰 - 2 - 羟乙基）- L - 半胱氨酸（GAMA）和N - 乙酰 - S - （苄基）- L - 半胱氨酸（BMA）的肌酐校正尿液水平均升高。在多元线性回归模型中，AD患者中BMA（甲苯代谢物）和DHBMA（1,3 - 丁二烯代谢物）的肌酐校正尿液水平似乎升高，尽管在多重比较校正后未达到统计学意义。此外，1,3 - 丁二烯和甲苯可刺激BMMCs脱颗粒，程度与化合物48/80相同。
一些VOC，如1,3 - 丁二烯和甲苯，可能与成人AD的发病机制有关。
