Institute of Biochemistry and Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, ON K1S 5B6, Canada.
Biomolecules. 2024 Jul 12;14(7):839. doi: 10.3390/biom14070839.
Epigenetic regulation, notably histone post-translational modification (PTM), has emerged as a major transcriptional control of gene expression during cellular stress adaptation. In the present study, we use an acid extraction method to isolate total histone protein and investigate dynamic changes in 23 well-characterized histone methylations/acetylations in the brains of wood frogs subject to 24-h freezing and subsequent 8-h thawed recovery conditions. Our results identify four histone PTMs (H2BK5ac, H3K14ac, H3K4me3, H3K9me2) and three histone proteins (H1.0, H2B, H4) that were significantly ( < 0.05) responsive to freeze-thaw in freeze-tolerant brains. Two other permissive modifications (H3R8me2a, H3K9ac) also trended downwards following freezing stress. Together, these data are strongly supportive of the proposed global transcriptional states of hypometabolic freeze tolerance and rebounded thawed recovery. Our findings shed light on the intricate interplay between epigenetic regulation, gene transcription and energy metabolism in wood frogs' adaptive response to freezing stress.
表观遗传调控，特别是组蛋白翻译后修饰（PTM），已成为细胞应激适应过程中基因表达的主要转录调控因子。在本研究中，我们使用酸提取法分离总组蛋白，并研究了在经历 24 小时冷冻和随后 8 小时解冻恢复条件下，木蛙脑中 23 种经过充分研究的组蛋白甲基化/乙酰化的动态变化。我们的结果确定了四种组蛋白 PTM（H2BK5ac、H3K14ac、H3K4me3、H3K9me2）和三种组蛋白（H1.0、H2B、H4），它们对冷冻耐受脑中的冻融有明显（<0.05）的反应。另外两种允许性修饰（H3R8me2a、H3K9ac）在受到冷冻应激后也呈下降趋势。这些数据强烈支持低代谢冷冻耐受和复温后恢复的拟议的整体转录状态。我们的发现揭示了在木蛙对冷冻应激的适应反应中，表观遗传调控、基因转录和能量代谢之间错综复杂的相互作用。
