Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou 510642, China.
Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Genes (Basel). 2024 Jun 21;15(7):823. doi: 10.3390/genes15070823.
OVATE family proteins (OFPs) are a class of plant-specific proteins with a conserved OVATE domain that play fundamental roles in fruit development and plant growth. Mango ( L.) is an economically important subtropical fruit tree characterized by a diverse array of fruit shapes and sizes. Despite extensive research on OFPs across various species, there remains a scarcity of information regarding OFPs in mango. Here, we have successfully identified 25 OFP genes (s) in mango, each of which exhibits the conserved OVATE domains. The gene exhibit a range of 2-6 motifs, with all genes containing both motif 1 and motif 2. Phylogenetic analysis on 97 OFPs (including 18 AtOFPs, 24 SlOFPs, 25 MiOFPs, and 30 OsOFPs) indicated that MiOFPs could be divided into three main clades: clade I, II, and III. Comparative morphological analysis identified significant variations in fruit longitudinal diameter, fruit transverse diameter, and fruit shape index between two distinct shaped mango cultivars ('Hongxiangya' and 'Jingpingmang') at DAP5, DAP7, and DAP10 stages. The subsequent examination of paraffin sections revealed distinct patterns of cell elongation. The majority of genes exhibited predominantly expressed in developing organs, specifically flowers and immature fruits, while displaying distinct expression patterns. RNA-Seq analysis revealed significant disparities in the expression levels of several genes, including 5, 11, 21, 22, 23, and 25, between the two mango cultivars. These findings suggest that these six genes may play a crucial role for fruit shape in mango, especially the 22. The findings of this study have established a basis for future investigations into s in mango, offering a solid foundation for further research in this field.
卵形家族蛋白 (OFPs) 是一类具有保守 OVATE 结构域的植物特异性蛋白，在果实发育和植物生长中起着重要作用。芒果 ( L.) 是一种重要的亚热带果树，其果实形状和大小多样。尽管在不同物种中对 OFPs 进行了广泛的研究，但关于芒果中的 OFPs 信息仍然很少。在这里，我们成功地在芒果中鉴定出了 25 个 OFP 基因 (s)，每个基因都具有保守的 OVATE 结构域。基因展示了 2-6 个基序的范围，所有基因都包含基序 1 和基序 2。对 97 个 OFPs (包括 18 个 AtOFPs、24 个 SlOFPs、25 个 MiOFPs 和 30 个 OsOFPs) 的系统发育分析表明，MiOFPs 可以分为三个主要分支：分支 I、II 和 III。比较形态分析在 DAP5、DAP7 和 DAP10 阶段，在两个不同形状的芒果品种 ('Hongxiangya' 和 'Jingpingmang') 之间发现了果实纵向直径、果实横向直径和果实形状指数的显著变化。随后对石蜡切片的检查显示出细胞伸长的明显模式。大多数基因主要在发育器官中表达，特别是花和未成熟果实，同时表现出不同的表达模式。RNA-Seq 分析显示，在两个芒果品种之间，包括 5、11、21、22、23 和 25 在内的几个基因的表达水平存在显著差异。这些发现表明，这六个基因可能在芒果果实形状中发挥关键作用，特别是 22。本研究的结果为今后对芒果中基因的研究奠定了基础，为该领域的进一步研究提供了坚实的基础。
