National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100097, China.
Int J Mol Sci. 2020 Feb 14;21(4):1293. doi: 10.3390/ijms21041293.
The shape of the tuberous root, a very important quality trait, varies dramatically among radish cultivars. Ovate family proteins () are plant-specific proteins that regulate multiple aspects of plant growth and development. To investigate the possible role of in radish tuberous root formation, 35 putative were identified from radish, and their expression patterns were detected during tuberous root development in six different radish cultivars. Phylogenetically, clustered together with and other members of this family that are known to regulate organ shape. Moreover, expression was negatively correlated with tuberous root elongation after the cortex splitting stage, which made this gene the top candidate for the involvement of tuberous root shape. To further characterize the function of , it was ectopically expressed in Arabidopsis. overexpression in Arabidopsis led to multiple phenotypical changes, especially the decreased length and increased width of the hypocotyl. Furthermore, expression was induced by all the five classic plant hormones except ethylene, and it was most sensitive to exogenous gibberellic acid treatment. We also found that was localized in the cytoplasm. Taken together, our results suggested the possible involvement for in suppressing radish tuberous root elongation and that it encodes a functional protein which mainly inhibits the elongation of Arabidopsis aerial organs.
块根的形状是一个非常重要的质量性状，在不同的萝卜品种中差异很大。卵形家族蛋白（）是植物特异性蛋白，调节植物生长和发育的多个方面。为了研究在萝卜块根形成过程中可能的作用，从萝卜中鉴定了 35 个假定的，并且在六个不同的萝卜品种的块根发育过程中检测了它们的表达模式。系统发育分析表明，与其他已知调节器官形状的家族成员一起聚类。此外，表达与皮层分裂后块根伸长呈负相关，这使得该基因成为参与块根形状的主要候选基因。为了进一步表征的功能，将其在拟南芥中异位表达。在拟南芥中过表达导致多种表型变化，特别是下胚轴的长度缩短和宽度增加。此外，除了乙烯外，所有五种经典植物激素都能诱导表达，它对外源赤霉素处理最敏感。我们还发现定位于细胞质中。综上所述，我们的结果表明在抑制萝卜块根伸长中可能涉及，并且它编码一个功能性蛋白，主要抑制拟南芥地上器官的伸长。
