School of Biological Science and Engineering, Hebei University of Science and Technology, Yuxiang Street 26, Shijiazhuang 050018, China.
Hebei Key Laboratory of Plant Genetic Engineering, Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China.
Genes (Basel). 2024 Jun 22;15(7):827. doi: 10.3390/genes15070827.
Nitrogen (N), as the main component of biological macromolecules, maintains the basic process of plant growth and development. GOGAT, as a key enzyme in the N assimilation process, catalyzes α-ketoglutaric acid and glutamine to form glutamate. In this study, six genes in wheat ( L.) were identified and classified into two subfamilies, () and (), according to the type of electron donor. Subcellular localization prediction showed that TaGOGAT3-D was localized in mitochondria and that the other five TaGOGATs were localized in chloroplasts. Via the analysis of promoter elements, many binding sites related to growth and development, hormone regulation and plant stress resistance regulations were found on the promoters. The tissue-specificity expression analysis showed that were mainly expressed in wheat leaves and flag leaves, while were highly expressed in roots and leaves. The expression level of and the enzyme activity of TaGOGAT3s in the leaves and roots of wheat seedlings were influenced by the treatment of N deficiency. This study conducted a systematic analysis of wheat genes, providing a theoretical basis not only for the functional analysis of , but also for the study of wheat nitrogen use efficiency (NUE).
氮（N）作为生物大分子的主要组成部分，维持着植物生长发育的基本过程。谷氨酸合酶（GOGAT）作为氮同化过程中的关键酶，催化α-酮戊二酸和谷氨酰胺形成谷氨酸。本研究在小麦中鉴定到 6 个基因，并根据电子供体的类型将其分为两个亚家族，（）和（）。亚细胞定位预测表明 TaGOGAT3-D 定位于线粒体，而其他五个 TaGOGATs 定位于叶绿体。通过启动子元件分析，在 启动子上发现了许多与生长发育、激素调节和植物抗逆性调节相关的结合位点。组织特异性表达分析表明， 主要在小麦叶片和旗叶中表达，而 在根和叶中高度表达。氮缺乏处理影响小麦幼苗叶片和根系中 和 TaGOGAT3s 的表达水平以及酶活性。本研究对小麦 基因进行了系统分析，不仅为 的功能分析提供了理论基础，也为研究小麦氮利用效率（NUE）提供了理论基础。
