College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
Genes (Basel). 2024 Jul 2;15(7):870. doi: 10.3390/genes15070870.
Ureide permeases (UPSs) mediate the transport of ureides, including allantoin and allantoate, which act as nitrogen-transporting compounds in plants and have recently been found to play a role in cellular signaling. To date, UPSs have not been reported in potato, and their identification is important for further function studies and for understanding molecular mechanisms of plant adverse responses. Based on potato genomic data, we identified 10 genes in potato ( L.). Then, we conducted a comprehensive study of the identified genes using bioinformatics methods. Genome phylogenetic and genomic localization analyses revealed that can be classified into four categories, are highly homologous to UPS members, and are distributed on three chromosomes. The six genes were investigated by RT-qPCR, and the findings indicated that all of these genes are involved in the response to several stresses, including low nitrogen, cold, ABA, salt, HO and drought. This study establishes a strong theoretical framework for investigating the function of potato genes, as well as the molecular mechanisms underlying the responses of these genes to various environmental stresses.
脲渗透酶（UPSs）介导包括尿囊素和尿囊酸在内的尿囊素的运输，它们在植物中作为氮转运化合物，最近被发现也在细胞信号转导中发挥作用。迄今为止，在马铃薯中尚未报道过 UPSs，它们的鉴定对于进一步的功能研究和理解植物不良反应的分子机制非常重要。基于马铃薯基因组数据，我们在马铃薯中鉴定了 10 个基因。然后，我们使用生物信息学方法对鉴定出的基因进行了全面研究。基因组系统发育和基因组定位分析表明，可以将其分为四类，与 UPS 成员高度同源，并分布在三条染色体上。通过 RT-qPCR 对 6 个基因进行了研究，结果表明这些基因均参与对几种胁迫的反应，包括低氮、低温、ABA、盐、HO 和干旱胁迫。这项研究为研究马铃薯基因的功能以及这些基因对各种环境胁迫的反应的分子机制奠定了坚实的理论框架。
