School of Dental Medicine, University of Belgrade, 11 000 Belgrade, Serbia.
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11 000 Belgrade, Serbia.
Int J Mol Sci. 2024 Jul 20;25(14):7940. doi: 10.3390/ijms25147940.
This study aimed to compare the biological properties of newly synthesized cements based on calcium phosphate with a commercially used cement, mineral trioxide aggregate (MTA). Strontium (Sr)-, Copper (Cu)-, and Zinc (Zn)-doped hydroxyapatite (miHAp) powder was obtained through hydrothermal synthesis and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectrometry (EDX). Calcium phosphate cement (CPC) was produced by mixing miHAp powder with a 20 wt.% citric acid solution, followed by the assessment of its compressive strength, setting time, and in vitro bioactivity. Acetylsalicylic acid (ASA) was added to the CPC, resulting in CPCA. Biological tests were conducted on CPC, CPCA, and MTA. The biocompatibility of the cement extracts was evaluated in vitro using human dental pulp stem cells (hDPSCs) and in vivo using a zebrafish model. Antibiofilm and antimicrobial effect (quantified by CFUs/mL) were assessed against and . None of the tested materials showed toxicity, while CPCA even increased hDPSCs proliferation. CPCA showed a better safety profile than MTA and CPC, and no toxic or immunomodulatory effects on the zebrafish model. CPCA exhibited similar antibiofilm effects against and to MTA.
本研究旨在比较基于磷酸钙的新型合成水泥与商业上使用的矿化三氧化物凝聚体（MTA）的生物学特性。通过水热合成获得锶（Sr）、铜（Cu）和锌（Zn）掺杂的羟基磷灰石（miHAp）粉末，并通过扫描电子显微镜（SEM）、X 射线衍射（XRD）和能谱（EDX）进行表征。通过将 miHAp 粉末与 20wt%柠檬酸溶液混合来制备磷酸钙水泥（CPC），然后评估其抗压强度、凝固时间和体外生物活性。在 CPC 中添加乙酰水杨酸（ASA），得到 CPCA。对 CPC、CPCA 和 MTA 进行生物学测试。使用人牙髓干细胞（hDPSCs）在体外和使用斑马鱼模型在体内评估水泥提取物的生物相容性。通过 CFU/mL 定量评估抗生物膜和抗菌效果（）。测试的材料均无毒性，而 CPCA 甚至增加了 hDPSCs 的增殖。CPCA 比 MTA 和 CPC 具有更好的安全性，对斑马鱼模型没有毒性或免疫调节作用。CPCA 对 和 表现出与 MTA 相似的抗生物膜作用。
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