磷酸钙骨水泥对人牙髓细胞生长和成牙本质细胞分化的影响。

Effect of calcium phosphate cements on growth and odontoblastic differentiation in human dental pulp cells.

机构信息

Department of Oral and Maxillofacial Pathology and Institute of Wonkwang Biomaterials Implant, School of Dentistry, Wonkwang University, Iksan, Korea.

出版信息

J Endod. 2010 Sep;36(9):1537-42. doi: 10.1016/j.joen.2010.04.027. Epub 2010 Jul 6.

DOI:10.1016/j.joen.2010.04.027

PMID:20728723

Abstract

OBJECTIVE

Calcium phosphate cements (CPCs) are an interesting class of bone substitute materials. However, the biological effects of CPCs have not been well studied in human dental pulp cells (HDPCs). The purpose of this study was to investigate the effects of CPCs on the mechanical properties, growth, and odontoblastic differentiation in HDPCs compared with Portland cement (PC) and mineral trioxide aggregate (MTA).

METHODS

Experimental CPCs either containing chitosan (Ch-CPC) or without chitosan (CPC) were composed from the alpha-tricalcium phosphate powder. Setting time, compressive strength measurements, cell growth, alkaline phosphatase (ALP) activity, the levels of messenger RNA for differentiation-related genes, and mineralization of the HDPCs on various cements were assessed.

RESULTS

The setting time for CPC-Ch was 7.5 minutes, which was significantly less than the 8.6 minutes for the CPC. On the seventh day of immersion, the compressive strength of CPC-CH reached 13.1 MPa, which was higher than 10.8 MPa of CPC. CPC and Ch-CPC-treated cells showed decreased cell proliferation but increased the levels of ALP activity, enhanced mineralized nodule formation, and upregulated odontoblastic markers messenger RNA including osteonectin, osteopontin, bone sialoprotein, dentin matrix protein-1, matrix extracellular phosphoglycoprotein, and dentin sialophosphoprotein (DSPP), compared with untreated control. The response of CPC and CP-CPC were similar to that of PC and MTA. However, the adhesion, growth, and differentiation in Ch-CPC-treated cells were similar to that in the CPC.

CONCLUSION

CPC may be useful for pulp-capping applications based on its abilities to promote HDPC differentiation.

摘要

目的

磷酸钙骨水泥（CPCs）是一类很有前途的骨替代材料。然而，CPCs 对人牙髓细胞（HDPCs）的生物学效应尚未得到充分研究。本研究旨在比较 CPCs 与波特兰水泥（PC）和三氧化矿物凝聚体（MTA）对 HDPCs 的机械性能、生长和成牙本质分化的影响。

方法

实验性 CPC 分别由壳聚糖（Ch-CPC）或无壳聚糖（CPC）组成，由 α-磷酸三钙粉末组成。测定凝固时间、抗压强度、细胞生长、碱性磷酸酶（ALP）活性、HDPCs 上各种水泥的分化相关基因的信使 RNA 水平和矿化。

结果

CPC-Ch 的凝固时间为 7.5 分钟，明显短于 CPC 的 8.6 分钟。在浸泡的第 7 天，CPC-CH 的抗压强度达到 13.1MPa，高于 CPC 的 10.8MPa。与未处理的对照组相比，CPC 和 Ch-CPC 处理的细胞增殖减少，但 ALP 活性增加，矿化结节形成增强，牙本质标志物信使 RNA 水平上调，包括骨桥蛋白、骨涎蛋白、骨唾液蛋白、牙本质基质蛋白-1、基质细胞外磷酸糖蛋白和牙本质涎磷蛋白（DSPP）。CPC 和 CP-CPC 的反应与 PC 和 MTA 相似。然而，Ch-CPC 处理的细胞的粘附、生长和分化与 CPC 相似。