De Mori Arianna, Karali Aikaterina, Daskalakis Evangelos, Hing Richard, Da Silva Bartolo Paulo Jorge, Cooper Glen, Blunn Gordon
School of Pharmacy and Biomedical Sciences, University of Portsmouth, St. Michael's Building, White Swan Road, Portsmouth PO1 2DT, UK.
Zeiss Global Centre, School of Mechanical and Design Engineering, University of Portsmouth, Portsmouth PO1 3DJ, UK.
Polymers (Basel). 2023 Dec 24;16(1):66. doi: 10.3390/polym16010066.
Large bone reconstruction following trauma poses significant challenges for reconstructive surgeons, leading to a healthcare burden for health systems, long-term pain for patients, and complex disorders such as infections that are difficult to resolve. The use of bone substitutes is suboptimal for substantial bone loss, as they induce localized atrophy and are generally weak, and unable to support load. A combination of strong polycaprolactone (PCL)-based scaffolds, with an average channel size of 330 µm, enriched with 20% / of hydroxyapatite (HA), β-tricalcium phosphate (TCP), or Bioglass 45S5 (Bioglass), has been developed and tested for bone regeneration in a critical-size ovine femoral condyle defect model. After 6 weeks, tissue ingrowth was analyzed using X-ray computed tomography (XCT), Backscattered Electron Microscopy (BSE), and histomorphometry. At this point, all materials promoted new bone formation. Histological analysis showed no statistical difference among the different biomaterials ( > 0.05), but PCL-Bioglass scaffolds enhanced bone formation in the center of the scaffold more than the other types of materials. These materials show potential to promote bone regeneration in critical-sized defects on load-bearing sites.
创伤后的大骨重建给重建外科医生带来了重大挑战，给卫生系统造成了医疗负担，给患者带来了长期疼痛，以及诸如难以解决的感染等复杂病症。对于大量骨质流失，骨替代物的使用并不理想，因为它们会导致局部萎缩，通常强度较弱，且无法承受负荷。一种基于聚己内酯（PCL）的强力支架已被开发并在临界尺寸的绵羊股骨髁缺损模型中进行骨再生测试，该支架平均通道尺寸为330微米，富含20%的羟基磷灰石（HA）、β-磷酸三钙（TCP）或生物活性玻璃45S5（生物玻璃）。6周后，使用X射线计算机断层扫描（XCT）、背散射电子显微镜（BSE）和组织形态计量学分析组织向内生长情况。此时，所有材料均促进了新骨形成。组织学分析显示不同生物材料之间无统计学差异（>0.05），但PCL-生物玻璃支架在支架中心比其他类型材料更能增强骨形成。这些材料显示出在承重部位的临界尺寸缺损中促进骨再生的潜力。
