Department of Chemical Engineering, University of Santiago, Chile, Avenida Libertador Bernardo O'Higgins 3363, Estación Central, Santiago 9170019, Chile.
Int J Mol Sci. 2024 Jul 20;25(14):7945. doi: 10.3390/ijms25147945.
The present study investigates the interactions between eight glucosinolate hydrolysis products (GHPs) sourced from broccoli by-products and the detoxifying enzymes of , namely eburicol 14-alpha-demethylase (CYP51) and glutathione-S-transferase (GST), through in silico analysis. Additionally, in vitro assays were conducted to explore the impact of these compounds on fungal growth. Our findings reveal that GHPs exhibit greater efficacy in inhibiting conidia germination compared to mycelium growth. Furthermore, the results demonstrate the antifungal activity of glucosinolate hydrolysis products derived from various parts of the broccoli plant, including inflorescences, leaves, and stems, against . Importantly, the results suggest that these hydrolysis products interact with the detoxifying enzymes of the fungus, potentially contributing to their antifungal properties. Extracts rich in GHPs, particularly iberin and indole-GHPs, derived from broccoli by-products emerge as promising candidates for biofungicidal applications, offering a sustainable and novel approach to plant protection by harnessing bioactive compounds from agricultural residues.
本研究通过计算机模拟分析了西兰花副产物中 8 种硫代葡萄糖苷水解产物（GHPs）与 解毒酶（即 eburicol 14-α-脱甲基酶（CYP51）和谷胱甘肽-S-转移酶（GST））之间的相互作用。此外，还进行了体外试验以研究这些化合物对真菌生长的影响。我们的研究结果表明，GHPs 在抑制分生孢子萌发方面比抑制菌丝生长更为有效。此外，结果表明，源自西兰花植物不同部位（包括花序、叶片和茎）的硫代葡萄糖苷水解产物对 具有抗真菌活性。重要的是，结果表明这些水解产物与真菌的解毒酶相互作用，这可能是它们具有抗真菌特性的原因。富含 GHPs 的提取物，特别是来自西兰花副产物的 Iberin 和吲哚-GHPs，有望成为生物杀菌应用的候选物，为利用农业残留物中的生物活性化合物来保护植物提供了一种可持续且新颖的方法。
