MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou 434025, China.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2024 Jul 21;25(14):7968. doi: 10.3390/ijms25147968.
The common bean ( L.) is an economically important food crop grown worldwide; however, its production is affected by various environmental stresses, including cold, heat, and drought stress. The plant U-box (PUB) protein family participates in various biological processes and stress responses, but the gene function and expression patterns of its members in the common bean remain unclear. Here, we systematically identified 63 U-box genes, including 8 tandem genes and 55 non-tandem genes, in the common bean. These genes were unevenly distributed across 11 chromosomes, with chromosome 2 holding the most members of the PUB family, containing 10 PUB genes. The analysis of the phylogenetic tree classified the 63 PUB genes into three groups. Moreover, transcriptome analysis based on cold-tolerant and cold-sensitive varieties identified 4 differentially expressed genes, suggesting their roles in cold tolerance. Taken together, this study serves as a valuable resource for exploring the functional aspects of the common bean U-box gene family and offers crucial theoretical support for the development of new cold-tolerant common bean varieties.
菜豆是一种在世界范围内广泛种植的重要经济作物；然而，其生产受到各种环境胁迫的影响，包括冷、热和干旱胁迫。植物 U -box（PUB）蛋白家族参与各种生物过程和应激反应，但该家族成员在菜豆中的基因功能和表达模式尚不清楚。在这里，我们系统地鉴定了 63 个 U-box 基因，包括 8 个串联基因和 55 个非串联基因，在菜豆中。这些基因不均匀地分布在 11 条染色体上，其中第 2 条染色体含有最多的 PUB 家族成员，包含 10 个 PUB 基因。基于耐寒和不耐寒品种的转录组分析鉴定出 4 个差异表达基因，表明它们在耐寒性中的作用。总之，这项研究为探索菜豆 U-box 基因家族的功能方面提供了有价值的资源，并为开发新的耐寒菜豆品种提供了重要的理论支持。
