Li Ruonan, Tang Jiandong, Li Jingjing, Wu Boxiao, Tang Junrong, Kan Huan, Zhao Ping, Zhang Yingjun, Wang Weihua, Liu Yun
Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, Kunming 650224, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650224, China.
Foods. 2024 Jul 18;13(14):2266. doi: 10.3390/foods13142266.
has important ornamental, medicinal, and food values, which also have tremendous potential for exploiting bioactivities. We performed the bioactivity-guided (antioxidant and antimicrobial) screening of eight fractions obtained from the ethyl acetate phase of . The antioxidant activity was measured by DPPH, ABTS, and FRAP, and the antibacterial activity was measured by the minimum inhibitory concentration (MIC) of , and . The results of bioactivity-guided isolation indicated that the major antioxidant compounds in the ethanolic extracts of may be present in fractions (Fr.) (A-G, obtained after silica gel column chromatography). Fr. (D-I, obtained after silica gel column chromatography) is a fraction of with antimicrobial activity. The structures of compounds were determined by spectral analysis and nuclear magnetic resonance (NMR) combined with the available literature on secondary metabolites of leaves. In this study, 17 compounds were identified, including four phenolics (, -, and ), a phenylpropane (), five terpenoids (-, , and ), four flavonoids and flavonoid glycosides (- and ), and two lignins ( and ). Compounds -, -, and were isolated from the genus for first time. The remaining compounds were also isolated from for first time. The evaluation of antioxidant and antimicrobial activities revealed that compounds , , , and exhibited higher antioxidant activity than the positive control drug (ascorbic acid), and compounds , , and showed similar activity to ascorbic acid. The other compounds had weaker or no significant antioxidant activities. The MIC of antibacterial activity for compounds , , and - against . was comparable to that of the positive control drug tetracycline at 125 µg/mL, and other secondary metabolites inhibited . and . at 250-500 µg/mL. This is also the first report of antioxidant and antimicrobial activities of compounds -, -, and . The results of the study enriched the variety of secondary metabolites of and laid the foundation for further research on the pharmacological efficacy and biological activity of this plant.
具有重要的观赏、药用和食用价值，在开发生物活性方面也具有巨大潜力。我们对从[具体植物名称]乙酸乙酯相获得的八个馏分进行了生物活性导向（抗氧化和抗菌）筛选。通过DPPH、ABTS和FRAP测定抗氧化活性，通过[具体细菌名称]、[具体细菌名称]和[具体细菌名称]的最低抑菌浓度（MIC）测定抗菌活性。生物活性导向分离结果表明，[具体植物名称]乙醇提取物中的主要抗氧化化合物可能存在于馏分（Fr.）（A - G，硅胶柱色谱后获得）中。Fr.（D - I，硅胶柱色谱后获得）是具有抗菌活性的[具体植物名称]馏分。通过光谱分析和核磁共振（NMR）结合关于[具体植物名称]叶次生代谢物的现有文献确定了化合物的结构。在本研究中，鉴定出17种化合物，包括四种酚类（[酚类化合物名称1]、[酚类化合物名称2]、[酚类化合物名称3]和[酚类化合物名称4]）、一种苯丙烷（[苯丙烷化合物名称]）、五种萜类（[萜类化合物名称1]、[萜类化合物名称2]、[萜类化合物名称3]、[萜类化合物名称4]和[萜类化合物名称5]）、四种黄酮类和黄酮苷（[黄酮类化合物名称1]和[黄酮类化合物名称2]）以及两种木质素（[木质素化合物名称1]和[木质素化合物名称2]）。化合物[化合物名称1]、[化合物名称2]和[化合物名称3]首次从[具体植物属名]中分离得到。其余化合物也首次从[具体植物名称]中分离得到。抗氧化和抗菌活性评估表明，化合物[化合物名称4]、[化合物名称5]、[化合物名称6]、[化合物名称7]和[化合物名称8]表现出比阳性对照药物（抗坏血酸）更高的抗氧化活性，化合物[化合物名称9]、[化合物名称10]和[化合物名称11]表现出与抗坏血酸相似的活性。其他化合物具有较弱的抗氧化活性或无显著抗氧化活性。化合物[化合物名称12]、[化合物名称13]和[化合物名称14]对[具体细菌名称]的抗菌活性MIC与阳性对照药物四环素在125 µg/mL时相当，其他次生代谢物在250 - 500 µg/mL时抑制[具体细菌名称1]和[具体细菌名称2]。这也是关于化合物[化合物名称15]、[化合物名称16]和[化合物名称17]抗氧化和抗菌活性的首次报道。该研究结果丰富了[具体植物名称]次生代谢物的种类，为进一步研究该植物的药理功效和生物活性奠定了基础。
