Hamet P, Sugimoto H, Umeda F, Lecavalier L, Franks D J, Orth D N, Chiasson J L
Metabolism. 1985 Dec;34(12 Suppl 1):25-31. doi: 10.1016/s0026-0495(85)80006-8.
Platelets are involved in homeostasis of the vascular wall at various levels. An important feature of this involvement is the potential for platelet proliferation. Platelets from normal subjects contain platelet-derived growth factor (PDGF), epithelial growth factor (EGF), and transforming growth factor. We have detected the presence of an excessive growth-promoting activity in the heated supernatant fraction derived from the platelets of young, insulin-dependent diabetics. This activity is most pronounced when measured in cultures of smooth muscle cells and fibroblasts. This activity may be further separated into cationic and anionic fractions by ion exchange chromatography of the platelet-rich supernatant. The cationic factor corresponds to PDGF, whereas the anionic factor appears to be identical to EGF. Chronic, intensive insulin therapy normalizes the excessive growth-promoting activity of platelets from diabetics. Further studies are needed to evaluate the differential release of those growth-promoting factors found in platelets of normal subjects and in patients with vascular disease.
血小板在血管壁的不同层面参与内环境稳定。这种参与的一个重要特征是血小板增殖的可能性。正常受试者的血小板含有血小板衍生生长因子（PDGF）、上皮生长因子（EGF）和转化生长因子。我们在来自年轻的胰岛素依赖型糖尿病患者血小板的加热上清液部分中检测到了过度的促生长活性。当在平滑肌细胞和成纤维细胞培养物中测量时，这种活性最为明显。通过对富含血小板的上清液进行离子交换色谱法，这种活性可进一步分离为阳离子和阴离子部分。阳离子因子对应于PDGF，而阴离子因子似乎与EGF相同。长期强化胰岛素治疗可使糖尿病患者血小板过度的促生长活性恢复正常。需要进一步研究来评估正常受试者和血管疾病患者血小板中发现的那些促生长因子的差异释放情况。
