Arai Y T
Microbiol Immunol. 1985;29(9):811-23. doi: 10.1111/j.1348-0421.1985.tb00884.x.
Chick embryo fibroblast-passaged Flury high egg passage (HEP) rabies virus failed to kill nude mice or cyclophosphamide-treated mice when inoculated intracerebrally. The virus regained neurovirulence for adult mice after three passages in mouse neuroblastoma C1300 cells (NA cells). However, even after 20 passages in NA cells, the virulence could not be increased to the level shown by the virus passaged several times in suckling mice. Some physiological and biological properties of the virus showing and not showing mouse virulence after five serial passages and after one single passage in NA cells, respectively, were compared.
鸡胚成纤维细胞传代的弗吕里高鸡胚传代（HEP）狂犬病病毒脑内接种时未能杀死裸鼠或经环磷酰胺处理的小鼠。该病毒在小鼠神经母细胞瘤C1300细胞（NA细胞）中传代三次后恢复了对成年小鼠的神经毒力。然而，即使在NA细胞中传代20次后，其毒力也无法提高到在乳鼠中传代几次后的病毒所显示的水平。分别比较了该病毒在NA细胞中连续传代五次和单次传代后显示和未显示小鼠毒力的一些生理和生物学特性。
