Barbin A, Besson F, Perrard M H, Béréziat J C, Kaldor J, Michel G, Bartsch H
Mutat Res. 1985 Nov-Dec;152(2-3):147-56. doi: 10.1016/0027-5107(85)90056-9.
Chloroethylene oxide (CEO), an ultimate carcinogenic metabolite of vinyl chloride, induces base-pair substitution mutations but not frameshift mutations in bacteria. The mutational specificity of CEO was investigated in Escherichia coli, using the trpA mutants developed by Yanofsky. Reversion frequencies to tryptophan prototrophy were analysed, and CEO was found to induce more GC----AT transitions than AT----TA transversions, in addition to a low frequency of other types of substitution. This specificity indicates that CEO is mutagenic through a miscoding DNA adduct. The results are discussed in relation to the various CEO-DNA adducts formed and to their reported or expected mispairing properties.
氯代环氧乙烷（CEO）是氯乙烯的一种最终致癌代谢产物，可诱导细菌发生碱基对置换突变，但不会导致移码突变。利用亚诺夫斯基开发的trpA突变体，在大肠杆菌中研究了CEO的突变特异性。分析了回复到色氨酸原养型的频率，发现CEO除了诱导低频率的其他类型置换外，还诱导更多的GC→AT转换而非AT→TA颠换。这种特异性表明CEO通过错误编码的DNA加合物具有致突变性。结合形成的各种CEO-DNA加合物及其报道的或预期的错配特性对结果进行了讨论。
