Nogueira J M, Garcia-de-Lomas J, Buesa F J, Prat J, Mir A, Camarena J J
Mycopathologia. 1985 Oct;92(1):31-5. doi: 10.1007/BF00442656.
Candida albicans colonization induces antibodies, which must be taken into account in the serological diagnosis of candidiasis. In order to determine the degree of this effect, an experimental study in rabbits free of specific anti-Candida antibodies by cutaneous and digestive inoculation has been carried out. The evolution of humoral response was studied over 8 weeks by indirect immunofluorescence (IIF), direct agglutination (DA), counterimmunoelectrophoresis (CIE) and double diffusion (DD). The cutaneous colonization detectable by culture was maintained until the second week in 70% of the animals and the presence of antibodies detectable by IIF and DA was observed after the 2nd week. The highest antibody titre by IFF and DA was 1/64, and was reached in the 5th week, with a tendency to drop in the following weeks. Precipitins were only detected by CIE in 15% of the animals in the 7th week. Elimination of yeast in stools continued only in 20% of the animals in the 2nd week of the experiment. Antibodies were detected by IIF and DA after the 2nd week, with the highest titres detectable by IFF in the 5th week. Precipitant antibodies detectable by CIE appeared in 15% of the animals in the 8th week.
白色念珠菌定植可诱导产生抗体，这在念珠菌病的血清学诊断中必须予以考虑。为了确定这种影响的程度，通过皮肤和消化道接种对无特异性抗念珠菌抗体的兔子进行了一项实验研究。通过间接免疫荧光法（IIF）、直接凝集法（DA）、对流免疫电泳法（CIE）和双向扩散法（DD）研究了8周内体液反应的演变。通过培养可检测到的皮肤定植在70%的动物中持续到第二周，第二周后观察到可通过IIF和DA检测到的抗体。IFF和DA检测到的最高抗体滴度为1/64，在第5周达到，随后几周有下降趋势。仅在第7周，15%的动物通过CIE检测到沉淀素。在实验的第二周，仅20%的动物粪便中的酵母菌被清除。第二周后通过IIF和DA检测到抗体，IFF检测到的最高滴度出现在第5周。第8周，15%的动物出现了可通过CIE检测到的沉淀抗体。
