Cui Wenzhuang, Gong Chu, Liu Yujie, Yue Ying, Wang Jun, Yang Zhizhou, Yang Junli
School of Materials Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
Shandong Laboratory of Advanced Materials and Green Manufacturing at Yantai, Yantai 264006, China.
Int J Biol Macromol. 2024 Oct;277(Pt 2):134208. doi: 10.1016/j.ijbiomac.2024.134208. Epub 2024 Jul 26.
Antibacterial hydrogels as burn wound dressings are capable of efficaciously defending against bacterial infection and accelerating burn wound healing. Thus far, a large plethora of antibacterial hydrogels have adopted numerous components and intricate preparation processes, yet restricting their practical industrialization applications. Simple and effective preparation methods of antibacterial hydrogels are hence urgently needed. Herein, an easy but efficacious strategy with the employment of two natural products pullulan and ε-poly-l-lysine (ε-PL) was designed to fabricate composite antibacterial hydrogels for burn wound healing for the first time. The hydrogel crosslinking networks were formed through amidation reactions between carboxylated pullulan derivative (CP) and ε-poly-l-lysine hydrochloride (ε-PL·HCl). The resulting hydrogels possessed high transparency, porous structures, tunable gelation time and gel content, relatively low swelling ratios, appropriate self-degradability, proper mechanical properties, strong in vitro bacteriostatic activities, non-cytotoxicity, capacities of facilitating cell migration and excellent hemocompatibility. In the infected burn model of mice, the hydrogels were observed to display prominent in vivo antibacterial activities and enable the acceleration of burn wound healing. We opine the simply and effectively prepared antibacterial hydrogels as promising dressings for burn wound recovery have broad industrialization prospects.
作为烧伤创面敷料的抗菌水凝胶能够有效抵御细菌感染并加速烧伤创面愈合。迄今为止，大量的抗菌水凝胶采用了众多成分和复杂的制备工艺，然而这限制了它们在实际工业化中的应用。因此，迫切需要简单有效的抗菌水凝胶制备方法。在此，首次设计了一种简单而有效的策略，即使用两种天然产物普鲁兰多糖和ε-聚-L-赖氨酸（ε-PL）来制备用于烧伤创面愈合的复合抗菌水凝胶。水凝胶交联网络是通过羧化普鲁兰多糖衍生物（CP）和ε-聚-L-赖氨酸盐酸盐（ε-PL·HCl）之间的酰胺化反应形成的。所得水凝胶具有高透明度、多孔结构、可调节的凝胶化时间和凝胶含量、相对较低的溶胀率、适当的自降解性、合适的机械性能、强大的体外抑菌活性、无细胞毒性、促进细胞迁移的能力以及优异的血液相容性。在小鼠感染性烧伤模型中，观察到水凝胶具有显著的体内抗菌活性，并能加速烧伤创面愈合。我们认为，这种简单有效制备的抗菌水凝胶作为烧伤创面恢复的有前景的敷料具有广阔的工业化前景。
