Department of Orthopaedic Surgery, Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310016, China.
Department of Orthopaedic Surgery, the Affiliated Hospital of Qingdao University, Qingdao, 266000, China.
J Nanobiotechnology. 2024 Jul 28;22(1):445. doi: 10.1186/s12951-024-02723-x.
The incidence of osteochondral defects caused by trauma, arthritis or tumours is increasing annually, but progress has not been made in terms of treatment methods. Due to the heterogeneous structure and biological characteristics of cartilage and subchondral bone, the integration of osteochondral repair is still a challenge.
In the present study, a novel bilayer hydrogel scaffold was designed based on anatomical characteristics to imitate superficial cartilage and subchondral bone. The scaffold showed favourable biocompatibility, and the addition of an antioxidant nanozyme (LiMnO) promoted reactive oxygen species (ROS) scavenging by upregulating antioxidant proteins. The cartilage layer effectively protects against chondrocyte degradation in the inflammatory microenvironment. Subchondral bionic hydrogel scaffolds promote osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) by regulating the AMPK pathway in vitro. Finally, an in vivo rat preclinical osteochondral defect model confirmed that the bilayer hydrogel scaffold efficiently promoted cartilage and subchondral bone regeneration.
In general, our biomimetic hydrogel scaffold with the ability to regulate the inflammatory microenvironment can effectively repair osteochondral defects. This strategy provides a promising method for regenerating tissues with heterogeneous structures and biological characteristics.
创伤、关节炎或肿瘤导致的骨软骨缺损的发病率逐年增加，但治疗方法方面并无进展。由于软骨和软骨下骨的结构和生物学特性具有异质性，骨软骨修复的整合仍然是一个挑战。
本研究基于解剖学特征设计了一种新型双层水凝胶支架，以模拟表层软骨和软骨下骨。该支架表现出良好的生物相容性，添加抗氧化纳米酶（LiMnO）通过上调抗氧化蛋白来促进活性氧（ROS）的清除。软骨层可有效防止炎症微环境中软骨细胞的降解。仿生软骨下骨水凝胶支架通过调节 AMPK 通路在体外促进大鼠骨髓间充质干细胞（BMSCs）的成骨分化。最后，体内大鼠临床前骨软骨缺损模型证实双层水凝胶支架可有效促进软骨和软骨下骨再生。
总之，我们具有调节炎症微环境能力的仿生水凝胶支架可有效修复骨软骨缺损。该策略为具有异质结构和生物学特性的组织再生提供了一种有前景的方法。
