Tran Lan Hai Anh, Lowe Lauren A, Deckel Yaam, Turner Matthew, Luong James, Khamis Omar Abdullah A, Amos Megan L, Wang Anna
School of Chemistry, UNSW, Sydney 2052, NSW, Australia.
Australian Centre for Astrobiology, UNSW, Sydney 2052, NSW, Australia.
ACS Phys Chem Au. 2024 May 20;4(4):400-407. doi: 10.1021/acsphyschemau.4c00011. eCollection 2024 Jul 24.
We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modeling the vesicle's scattering in a microscope with a Lorenz-Mie light scattering model and comparing the results to data holograms, we are able to measure the vesicle's refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables the retrieval of vesicle loading for nanoscale vesicles.
我们报告了使用在线数字全息显微镜对细胞大小的脂质囊泡负载量进行量化的工作。该方法不需要荧光报告分子、荧光示踪剂或放射性示踪剂。单色LED光源取代传统照明来生成全息图而非明场图像。通过用洛伦兹-米氏光散射模型对显微镜中囊泡的散射进行建模，并将结果与数据全息图进行比较，我们能够测量囊泡的折射率，进而测量其负载量。对体光散射测量进行同样的比较，能够获取纳米级囊泡的负载量。
