Ruffner David B, Cheong Fook Chiong, Blusewicz Jaroslaw M, Philips Laura A
Opt Express. 2018 May 14;26(10):13239-13251. doi: 10.1364/OE.26.013239.
Micrometer sized particles can be accurately characterized using holographic video microscopy and Lorenz-Mie fitting. In this work, we explore some of the limitations in holographic microscopy and introduce methods for increasing the accuracy of this technique with the use of multiple wavelengths of laser illumination. Large high index particle holograms have near degenerate solutions that can confuse standard fitting algorithms. Using a model based on diffraction from a phase disk, we explain the source of these degeneracies. We introduce multiple color holography as an effective approach to distinguish between degenerate solutions and provide improved accuracy for the holographic analysis of sub-visible colloidal particles.
微米级颗粒可以使用全息视频显微镜和洛伦兹-米氏拟合进行精确表征。在这项工作中，我们探讨了全息显微镜的一些局限性，并介绍了使用多波长激光照明提高该技术精度的方法。大型高折射率颗粒全息图具有近乎简并的解，这可能会使标准拟合算法产生混淆。使用基于相位盘衍射的模型，我们解释了这些简并性的来源。我们引入多色全息术作为区分简并解的有效方法，并为亚可见胶体颗粒的全息分析提供更高的精度。
