Scott Haden L, Kennison Kristen B, Enoki Thais A, Doktorova Milka, Kinnun Jacob J, Heberle Frederick A, Katsaras John
Large Scale Structures Group, Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
Shull-Wollan Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
Symmetry (Basel). 2021 Aug;13(8). doi: 10.3390/sym13081356. Epub 2021 Jul 26.
It is well known that the lipid distribution in the bilayer leaflets of mammalian plasma membranes (PMs) is not symmetric. Despite this, model membrane studies have largely relied on chemically symmetric model membranes for the study of lipid-lipid and lipid-protein interactions. This is primarily due to the difficulty in preparing stable, asymmetric model membranes that are amenable to biophysical studies. However, in the last 20 years, efforts have been made in producing more biologically faithful model membranes. Here, we review several recently developed experimental and computational techniques for the robust generation of asymmetric model membranes and highlight a new and particularly promising technique to study membrane asymmetry.
众所周知，哺乳动物质膜（PMs）双层小叶中的脂质分布是不对称的。尽管如此，模型膜研究在很大程度上依赖于化学对称的模型膜来研究脂质-脂质和脂质-蛋白质相互作用。这主要是由于制备适用于生物物理研究的稳定、不对称模型膜存在困难。然而，在过去20年里，人们一直在努力生产更具生物学真实性的模型膜。在这里，我们回顾了几种最近开发的用于稳健生成不对称模型膜的实验和计算技术，并强调了一种新的、特别有前景的研究膜不对称性的技术。
