与热灭活C1q结合的循环免疫复合物样物质会干扰免疫复合物的C1q固相测定。
Circulating immune complex-like materials which bind to heat inactivated C1q interfere with the C1q solid phase assay for immune complexes.
作者信息
Fukuda K, Seino J, Kinoshita Y, Sudo K, Horigome I, Saito T, Furuyama T, Yoshinaga K
出版信息
Tohoku J Exp Med. 1985 Aug;146(4):449-56. doi: 10.1620/tjem.146.449.
C1q solid phase assay (C1q SP) was devised based on the fact that immune complexes (IC) and aggregated human globulin (AHG) bind to C1q. Neither IC nor AHG was found to bind to heat inactivated C1q. On the other hand, circulating immune complex (CIC)-like materials in patients' sera were able to bind to heat inactivated C1q, indicating that these CIC-like materials are not true CIC. Gel filtration analysis showed that molecular size of such CIC-like materials was almost the same as monomeric IgG, while true CIC were in heavy fractions. True CIC did not bind to heat inactivated C1q but bind only to native C1q. The CIC-like activity is not due to rheumatoid factors. About 2/3 of CIC positive sera by C1q-SP are not really CIC positive but are due to interference by the CIC-like materials.
C1q固相测定法（C1q SP）是基于免疫复合物（IC）和聚合人球蛋白（AHG）与C1q结合这一事实而设计的。未发现IC和AHG与热灭活的C1q结合。另一方面，患者血清中循环免疫复合物（CIC）样物质能够与热灭活的C1q结合，这表明这些CIC样物质并非真正的CIC。凝胶过滤分析显示，此类CIC样物质的分子大小与单体IgG几乎相同，而真正的CIC则存在于重馏分中。真正的CIC不与热灭活的C1q结合，而仅与天然C1q结合。CIC样活性并非由类风湿因子引起。通过C1q-SP检测呈CIC阳性的血清中，约2/3并非真正的CIC阳性，而是由于CIC样物质的干扰所致。
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