Guarnieri Renzo, Reda Rodolfo, Di Nardo Dario, Miccoli Gabriele, Pagnoni Francesco, Zanza Alessio, Testarelli Luca
Private Practice, Treviso, Italy.
Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Rome, Italy.
J Dent Res Dent Clin Dent Prospects. 2024 Spring;18(2):135-142. doi: 10.34172/joddd.40958. Epub 2024 Jun 24.
This study evaluated the gingival crevicular fluid (GCF) and Peri- implant crevicular fluid (PICF) concentrations of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and active metalloproteinase-8 (a-MMP-8) in sites with healthy conditions vs. sites affected by periodontitis (PER) and peri-implantitis (PIM).
Periodontally healthy (PH) sites with PER, sites with peri-implant health (PIH), and sites with PIM were investigated intra-individually, according to the inclusion criteria of each group. Probing pocket depth (PPD), plaque index, gingival index, and the presence or absence of bleeding on probing (BoP) were evaluated. In GCF and PICF samples, IL-1β, IL-6, and TNF-α were quantified by ELISA Duoset® kit in combination with Ultramark® micro-ELISA digital reader; a-MMP8 concentration was analyzed by a chairside test (Perio/ImplantSafe®) in combination with a digital reader (ORALyzer®).
The concentrations of IL-6 and IL-1β, TNF-α, and a-MMP-8 were significantly higher in the PIM and PER sites compared to healthy sites (<0.05). Significantly higher concentrations of IL-1β and a-MMP-8 were found in PIM vs. PER sites (<0.05), while the concentrations of IL-6 and TNF-α did not differ between the PIM and PER groups (>0.05).
aMMP-8, IL-6, IL-1β, and TNF-α presented higher GCF/PICF concentrations in diseased periodontal and peri-implant sites. However, only the concentrations of IL-1β and a-MMP-8 were significantly higher in PIM than in PER sites.
本研究评估了在健康状况的部位与受牙周炎（PER）和种植体周围炎（PIM）影响的部位中，龈沟液（GCF）和种植体周围龈沟液（PICF）中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和活性金属蛋白酶-8（a-MMP-8）的浓度。
根据每组的纳入标准，对患有牙周炎（PER）的牙周健康（PH）部位、种植体周围健康（PIH）部位和患有种植体周围炎（PIM）的部位进行个体内研究。评估探诊深度（PPD）、菌斑指数、牙龈指数以及探诊出血（BoP）情况。在GCF和PICF样本中，IL-1β、IL-6和TNF-α通过ELISA Duoset®试剂盒结合Ultramark®微型ELISA数字读数仪进行定量；a-MMP8浓度通过椅旁检测（Perio/ImplantSafe®）结合数字读数仪（ORALyzer®）进行分析。
与健康部位相比，PIM和PER部位的IL-6、IL-1β、TNF-α和a-MMP-8浓度显著更高（<0.05）。在PIM与PER部位相比，IL-1β和a-MMP-8的浓度显著更高（<0.05），而PIM和PER组之间IL-6和TNF-α的浓度没有差异（>0.05）。
aMMP-8、IL-6、IL-1β和TNF-α在患病的牙周和种植体周围部位呈现出更高的GCF/PICF浓度。然而，只有IL-1β和a-MMP-8的浓度在PIM部位显著高于PER部位。
