Department of Oral and Maxillofacial Surgery, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Center for Dentistry and Oral Hygiene, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
J Clin Periodontol. 2021 Apr;48(4):590-601. doi: 10.1111/jcpe.13423. Epub 2021 Feb 16.
To compare biomarker levels in peri-implant crevicular fluid (PICF) of healthy implants with levels in PICF of implants with peri-implantitis (before and after non-surgical treatment).
Samples were taken from 20 healthy implants (n = 17 patients) and from 20 implants with peri-implantitis (n = 19 patients) before and 3 months after non-surgical treatment using the Airflow Master Piezon (EMS). A Luminex™ assay was used to evaluate pro-inflammatory and anti-inflammatory cytokines IL-1β, TNF-α, IL-6 and G-CSF, collagen degradation enzyme MMP-8, chemokines MCP-1 & MIP-1α/CCL3, bone markers OPG and sRANKL and interferon-γ. Clinical and radiographical characteristics were assessed. A Mann-Whitney U and Wilcoxon signed-rank test analysed between- and within-group differences.
IL-1β and MMP-8 levels were found significantly elevated in implants with peri-implantitis (p = .007; p = <.001, respectively). No difference in levels of TNF-α, IL-6, MCP-1 and MIP-1α/CCL3, OPG and G-CSF between healthy and diseased implants was found. Levels of sRANKL and INF-γ were under the level of detection. None of the biomarker levels improved after non-surgical therapy, and levels of IL-1β and MMP-8 remained high.
Implants diagnosed with peri-implantitis have higher levels of IL-1β and MMP-8 in PICF compared to healthy implants. Non-surgical therapy did not influence the inflammatory immune response.
比较健康种植体周围黏膜龈沟液（PICF）和种植体周围炎（非手术治疗前后）种植体周围黏膜龈沟液中的生物标志物水平。
使用 Airflow Master Piezon（EMS）从 20 个健康种植体（n = 17 例患者）和 20 个种植体周围炎种植体（n = 19 例患者）中采集样本。使用 Luminex™ assay 评估促炎和抗炎细胞因子 IL-1β、TNF-α、IL-6 和 G-CSF、胶原降解酶 MMP-8、趋化因子 MCP-1 和 MIP-1α/CCL3、骨标志物 OPG 和 sRANKL 和干扰素-γ。评估临床和影像学特征。采用 Mann-Whitney U 和 Wilcoxon 符号秩检验分析组间和组内差异。
发现种植体周围炎种植体中 IL-1β 和 MMP-8 水平显著升高（p = 0.007；p < 0.001）。健康和患病种植体之间 TNF-α、IL-6、MCP-1 和 MIP-1α/CCL3、OPG 和 G-CSF 的水平没有差异。sRANKL 和 INF-γ的水平低于检测水平。非手术治疗后，没有一种生物标志物水平得到改善，IL-1β 和 MMP-8 水平仍然较高。
与健康种植体相比，诊断为种植体周围炎的种植体 PICF 中 IL-1β 和 MMP-8 水平更高。非手术治疗并未影响炎症免疫反应。
