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开放式喷雾机器人：一种用于疾病筛查的高通量纸喷雾质谱平台。

The Open SprayBot: A high-throughput paper spray mass spectrometry platform for disease screening.

作者信息

García-Rojas Nancy Shyrley, Guillén-Alonso Héctor, MacKay Scott, Torres-Calzada Claudia, Soto-Rodriguez Leonardo Daniel, Winkler Robert, Wishart David S

机构信息

Laboratorio de Análisis Bioquímico e Instrumental, Unidad de Genómica Avanzada, Cinvestav, Km. 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, Guanajuato, México.

Department of Biological Science, University of Alberta, Edmonton, Canada.

出版信息

HardwareX. 2024 Jun 30;19:e00551. doi: 10.1016/j.ohx.2024.e00551. eCollection 2024 Sep.

DOI:10.1016/j.ohx.2024.e00551
PMID:39071222
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11283016/
Abstract

Newborn disease screening increases survival, improves quality of life and reduces treatment costs for healthcare systems. Mass spectrometry (MS) is an effective method for metabolic screening. However, conventional analytical methods require biofluid handling and cooling conditions during transport, making the logistics difficult and expensive, especially for remote regions. 'Paper-spray' (PS) ionization generates a charged solvent spray from samples deposited on paper strips. Therefore, samples can be applied on a suitable matrix and shipped as dried spots to diagnostic laboratories with standard postal or messenger services. We built a robotic platform, the 'Open SprayBot', to automatically analyze paper-deposited samples via PS-MS and increase the sample throughput. The system is operated via RUMBA32 and Arduino Mega boards. A commercial syringe pump and power supply provide solvent application and electrical current required for PS-MS. The usability of the Open SprayBot was demonstrated by quantifying palmitoyl-l-carnitine, a common biomarker in newborn screening.

摘要

新生儿疾病筛查可提高生存率、改善生活质量并降低医疗系统的治疗成本。质谱分析法（MS）是一种有效的代谢筛查方法。然而，传统分析方法在运输过程中需要对生物流体进行处理并保持冷却条件，这使得物流变得困难且成本高昂，尤其是对于偏远地区而言。“纸喷雾”（PS）电离可从沉积在纸条上的样品中产生带电溶剂喷雾。因此，样品可以涂覆在合适的基质上，并作为干斑通过标准邮政或信使服务运送到诊断实验室。我们构建了一个机器人平台“开放式喷雾机器人”，通过PS-MS自动分析纸沉积样品并提高样品通量。该系统通过RUMBA32和Arduino Mega板运行。一个商用注射泵和电源提供PS-MS所需的溶剂施加和电流。通过定量棕榈酰-L-肉碱（新生儿筛查中的一种常见生物标志物）证明了开放式喷雾机器人的可用性。

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