Umlauf Frederik, Diebolt Coline M, Englisch Colya N, Flockerzi Fidelis, Tschernig Thomas
Institute of Anatomy and Cell Biology, Saarland University, Faculty of Medicine, D-66421 Homburg, Germany.
Institute of Pathology, Saarland University, Faculty of Medicine, D-66421 Homburg, Germany.
Exp Ther Med. 2024 Jul 12;28(3):363. doi: 10.3892/etm.2024.12652. eCollection 2024 Sep.
Transient receptor potential channel canonical 5 (TRPC5) is a non-selective ion channel; ion influx through TRPC5 causes activation of downstream signaling pathways. In addition, TRPC5 has been identified as having a potential role in pathological processes, particularly in diseases caused by cellular cation homeostasis dysregulation, such as bronchial asthma or pulmonary hypertension. However, the expression and distribution of TRPC5 in the human lung remain unclear. To date, TRPC5 has only been detected in a few cell types in the human lung, such as airway, pulmonary venous and arterial smooth muscle cells. The present study therefore aimed to investigate the protein expression of TRPC5 in the human lung and to evaluate its histological distribution. Human lung samples were obtained from six preserved body donors. After processing, both hematoxylin & eosin staining, as well as immunohistochemistry were performed. Microscopic analysis revealed medium to strong immunostaining signals in all lung structures examined, including the pleura, pulmonary arteries and veins, bronchioles, alveolar septa, type 1 and 2 pneumocytes, as well as alveolar macrophages. Current research suggests that TRPC5 may be involved in various pathological processes in the human lung and some pharmacological compounds have already been identified that affect the function of TRPC5. Therefore, TRPC5 may present a novel drug target for therapeutic intervention in various lung diseases. The results of the present study indicate that the TRPC5 protein is expressed in all examined histological structures of the human lung. These findings suggest that TRPC5 may be more important for physiological cell function and pathophysiological cell dysfunction in the lung than is currently known. Further research is needed to explore the role and therapeutic target potential of TRPC5 in the human lung.
瞬时受体电位通道蛋白5（TRPC5）是一种非选择性离子通道；通过TRPC5的离子内流会导致下游信号通路的激活。此外，TRPC5已被确定在病理过程中具有潜在作用，尤其是在由细胞阳离子稳态失调引起的疾病中，如支气管哮喘或肺动脉高压。然而，TRPC5在人肺中的表达和分布仍不清楚。迄今为止，仅在人肺的少数细胞类型中检测到TRPC5，如气道、肺静脉和动脉平滑肌细胞。因此，本研究旨在调查TRPC5在人肺中的蛋白表达并评估其组织学分布。从六名遗体捐赠者处获取人肺样本。经过处理后，进行了苏木精-伊红染色以及免疫组织化学检测。显微镜分析显示，在所检查的所有肺结构中，包括胸膜、肺动脉和静脉、细支气管、肺泡隔、1型和2型肺细胞以及肺泡巨噬细胞，均有中度至强免疫染色信号。目前的研究表明，TRPC5可能参与人肺中的各种病理过程，并且已经确定了一些影响TRPC5功能的药理化合物。因此，TRPC5可能是各种肺部疾病治疗干预的新型药物靶点。本研究结果表明，TRPC5蛋白在人肺所有检查的组织结构中均有表达。这些发现表明，TRPC5对肺中生理细胞功能和病理生理细胞功能障碍可能比目前所知更为重要。需要进一步研究以探索TRPC5在人肺中的作用和治疗靶点潜力。
