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重视体外胚胎模型操作的标准化。

Importance of In Vitro Embryo Model Procedure Standardization.

机构信息

Department of Histology and Embryology, Faculty of Medicine, Ege University, İzmir, Türkiye.

Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Zentrum München, Germany.

出版信息

J Clin Lab Anal. 2024 Jul;38(13-14):e25082. doi: 10.1002/jcla.25082. Epub 2024 Jul 29.

Abstract

In vivo studies offer a detailed understanding of organism functioning, surpassing the insights provided by in vitro studies. These experiments are crucial for comprehending disease emergence, progression, and associated mechanisms in humans, as well as for developing treatments. When choosing experimental models, factors such as genomic similarity, physiological relevance, ethical appropriateness, and economic feasibility must be considered. Standardized protocols enhance the reliability, and reproducibility of scientific methods, promoting the assessment of research in the scientific literature. Researchers conducting embryo studies should establish and document standardized protocols for increased data comparability. Standardization is vital for scientific validity, reproducibility, and comparability in both in vivo and in vitro studies, ensuring the accuracy and reliability of experimental results and advancing scientific knowledge.

摘要

体内研究提供了对生物体功能的详细了解,超越了体外研究提供的见解。这些实验对于理解人类疾病的出现、进展和相关机制以及开发治疗方法至关重要。在选择实验模型时,必须考虑基因组相似性、生理相关性、伦理适宜性和经济可行性等因素。标准化方案提高了科学方法的可靠性和可重复性,促进了科学文献中研究的评估。进行胚胎研究的研究人员应建立和记录标准化方案,以提高数据可比性。标准化对于体内和体外研究的科学有效性、可重复性和可比性至关重要,确保实验结果的准确性和可靠性,并推动科学知识的发展。

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本文引用的文献

1
Stem cells used to model a two-week-old human embryo.用于模拟两周大人类胚胎的干细胞。
Nature. 2023 Oct;622(7983):469-470. doi: 10.1038/d41586-023-03150-y.
3
Self-patterning of human stem cells into post-implantation lineages.人类干细胞自我成形成植后谱系。
Nature. 2023 Oct;622(7983):574-583. doi: 10.1038/s41586-023-06354-4. Epub 2023 Jun 27.
4
Pluripotent stem cell-derived model of the post-implantation human embryo.多能干细胞衍生的人类着床后胚胎模型。
Nature. 2023 Oct;622(7983):584-593. doi: 10.1038/s41586-023-06368-y. Epub 2023 Jun 27.
6
Carnegie in 4D? Stem-cell-based models of human embryo development.4D 版卡内基?基于干细胞的人类胚胎发育模型。
Semin Cell Dev Biol. 2022 Nov;131:44-57. doi: 10.1016/j.semcdb.2022.05.023. Epub 2022 Jun 11.

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