College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, PR China.
College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006479.
A Gram-stain-positive, rod-shaped, non-spore-forming and non-motile bacterium, designated strain WY-16. Growth was observed at 20-42 °C (optimum, 30 °C), pH 6-9 (optimum, pH 7) and salinity of 0-3 % (w/v; optimum, 1 %). Phylogenetic analysis based on genome sequences indicated that WY-16 was affiliated to the family and most closely related to and . The average nucleotide identity values between strain WY-16 and and were 74.7 and 72.5 %, respectively. The digital DNA-DNA hybridization values between strain WY-16 and and were 19.6 and 18.6 %, respectively. The predominant fatty acids were anteiso-C, iso-C and iso-C 10-methyl. The major menaquinones were MK-12, MK-13, MK-14 and MK-15. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified glycolipid and one unidentified phospholipid. The cell-wall peptidoglycan contained 2,4-diaminobutyric acid as the diamino acid and ribose, rhamnose, glucose and galactose were the major cell-wall sugars. Based on phenotypic, genotypic and phylogenetic evidence, strain WY-16 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is WY-16 (=GDMCC 1.4011=JCM 36421).
一株革兰氏阳性、杆状、不产芽孢、不运动的细菌，命名为WY-16 菌株。该菌在 20-42°C（最佳温度 30°C）、pH6-9（最佳 pH 值 7）和 0-3%（w/v；最佳 1%）盐度下生长。基于基因组序列的系统发育分析表明，WY-16 菌株属于 科，与 和 最为密切相关。WY-16 菌株与 和 之间的平均核苷酸同一性值分别为 74.7%和 72.5%。WY-16 菌株与 和 之间的数字 DNA-DNA 杂交值分别为 19.6%和 18.6%。主要脂肪酸为 anteiso-C、iso-C 和 iso-C 10-methyl。主要的menaquinones 为 MK-12、MK-13、MK-14 和 MK-15。主要的极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的糖脂和一种未鉴定的磷脂。细胞壁肽聚糖含有 2,4-二氨基丁酸作为二氨基酸，核糖、鼠李糖、葡萄糖和半乳糖是主要的细胞壁糖。基于表型、基因型和系统发育证据，WY-16 菌株代表了 属中的一个新种，提议将其命名为 sp. nov.。模式菌株为 WY-16（=GDMCC 1.4011=JCM 36421）。
