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sp. nov.,分离自湖滨土壤。

sp. nov., isolated from lakeside soil.

机构信息

College of Life Sciences, Jiangxi Normal University, Nanchang, Jiangxi 330022, PR China.

College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.

出版信息

Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006479.

Abstract

A Gram-stain-positive, rod-shaped, non-spore-forming and non-motile bacterium, designated strain WY-16. Growth was observed at 20-42 °C (optimum, 30 °C), pH 6-9 (optimum, pH 7) and salinity of 0-3 % (w/v; optimum, 1 %). Phylogenetic analysis based on genome sequences indicated that WY-16 was affiliated to the family and most closely related to and . The average nucleotide identity values between strain WY-16 and and were 74.7 and 72.5 %, respectively. The digital DNA-DNA hybridization values between strain WY-16 and and were 19.6 and 18.6 %, respectively. The predominant fatty acids were anteiso-C, iso-C and iso-C 10-methyl. The major menaquinones were MK-12, MK-13, MK-14 and MK-15. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified glycolipid and one unidentified phospholipid. The cell-wall peptidoglycan contained 2,4-diaminobutyric acid as the diamino acid and ribose, rhamnose, glucose and galactose were the major cell-wall sugars. Based on phenotypic, genotypic and phylogenetic evidence, strain WY-16 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is WY-16 (=GDMCC 1.4011=JCM 36421).

摘要

一株革兰氏阳性、杆状、不产芽孢、不运动的细菌,命名为WY-16 菌株。该菌在 20-42°C(最佳温度 30°C)、pH6-9(最佳 pH 值 7)和 0-3%(w/v;最佳 1%)盐度下生长。基于基因组序列的系统发育分析表明,WY-16 菌株属于 科,与 和 最为密切相关。WY-16 菌株与 和 之间的平均核苷酸同一性值分别为 74.7%和 72.5%。WY-16 菌株与 和 之间的数字 DNA-DNA 杂交值分别为 19.6%和 18.6%。主要脂肪酸为 anteiso-C、iso-C 和 iso-C 10-methyl。主要的menaquinones 为 MK-12、MK-13、MK-14 和 MK-15。主要的极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的糖脂和一种未鉴定的磷脂。细胞壁肽聚糖含有 2,4-二氨基丁酸作为二氨基酸,核糖、鼠李糖、葡萄糖和半乳糖是主要的细胞壁糖。基于表型、基因型和系统发育证据,WY-16 菌株代表了 属中的一个新种,提议将其命名为 sp. nov.。模式菌株为 WY-16(=GDMCC 1.4011=JCM 36421)。

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