Key Laboratory of System Bioengineering, Ministry of Education, Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
Frontier Science Center of the Ministry of Education, Tianjin University, Tianjin 300072, People's Republic of China.
J Agric Food Chem. 2024 Aug 7;72(31):17166-17175. doi: 10.1021/acs.jafc.4c02728. Epub 2024 Jul 29.
Methionine is the only nonpolar α-amino acid containing sulfur among the eight essential amino acids and is closely related to the metabolism of sulfur-containing compounds in the human body. Widely used in feed, medicine, food, and other fields, the market demand is increasing annually. However, low productivity and high cost largely limit the industrial production of methionine, and many novel production methods still have their own disadvantages. In this paper, the available methods for synthesizing methionine are reviewed and discussed. The latest strategies for improving methionine production are further introduced, including culture medium optimization, mutation technology, expression of key genes in the metabolic pathway, knockout and recombination, as well as the engineering of membrane transporters, the fermentation-enzymatic coupling route, and innovation of CO biotransformation.
蛋氨酸是 8 种必需氨基酸中唯一含有硫的非极性α-氨基酸，与人体内含硫化合物的代谢密切相关。广泛应用于饲料、医药、食品等领域，市场需求量逐年增加。但由于生产效率低、成本高，很大程度上限制了蛋氨酸的工业生产，许多新型生产方法仍存在各自的缺点。本文对合成蛋氨酸的现有方法进行了综述和讨论。进一步介绍了提高蛋氨酸生产的最新策略，包括培养基优化、突变技术、代谢途径中关键基因的表达、敲除和重组，以及膜转运蛋白的工程改造、发酵-酶耦联途径和 CO 生物转化的创新。
