Mao Lisi, Han Xionggao, Wu Honghua, Xu Lingyan, Zheng Hui, Wang Fengping, Wang Chaoyue, Xu Jingyan
Department of Pharmacy, Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, 324000, China.
Jinhua Institute of Zhejiang University, Jinhua, 321000, China.
J Fluoresc. 2025 Feb 4. doi: 10.1007/s10895-024-04083-z.
Herein, an imidazo-pyridin-derived fluorescent probe ImPy-HS was developed for monitoring the hydrogen sulfide (HS) level in the water decoction of Chinese medicinal materials. Under the excitation at 355 nm, ImPy-HS exhibited the obvious fluorescence response at 490 nm. The detection system with ImPy-HS showed the advantages including relatively high sensitivity, high selectivity, and high steadiness. Especially, in the water decoction of Chinese medicinal materials with HS from both addition and excessive fumigation then reduction, ImPy-HS achieved the monitoring of the HS level. ImPy-HS also realized the imaging of both exogenous and endogenous HS as well as the water decoction-treated affection. The information here was meaningful for studying the quality control of Chinese medicinal materials.
在此，开发了一种咪唑并吡啶衍生的荧光探针ImPy-HS，用于监测中药材水煎液中的硫化氢（HS）水平。在355nm激发下，ImPy-HS在490nm处表现出明显的荧光响应。含有ImPy-HS的检测系统具有灵敏度较高、选择性高和稳定性高的优点。特别是，在添加HS以及过度熏蒸然后还原的中药材水煎液中，ImPy-HS实现了对HS水平的监测。ImPy-HS还实现了外源性和内源性HS的成像以及水煎液处理后的影响。此处的信息对于研究中药材的质量控制具有重要意义。
