Al-Dabbagh Sahad M K, Alseady Haider H, Alhadad Enas J
Department of Medical Laboratory Techniques, Institute of Medical Technology Al-Mansour, Middle Technical University, Baghdad, Iraq.
Department of Medical Laboratory Techniques, Technical Institute of Babylon, Al-Furat Al-Awsat Technical University, Babylon, Iraq.
Vet World. 2024 Jun;17(6):1348-1355. doi: 10.14202/vetworld.2024.1348-1355. Epub 2024 Jun 21.
A total of 10% of the global population succumbs to amoebiasis yearly, equating to 50,000-100,000 recorded fatalities. It is closely associated with contaminated food and water supplies due to human feces. The disease's pathophysiology remains a subject of ongoing debate among experts. Some experts attribute the role of the host's conditions, parasite species and strain, and infection intensity in eliciting clinical symptoms. The aim of this study was to perform molecular identification of species isolated from humans and cattle.
Stool samples from three hundred patients and one hundred cattle were collected from different regions, age groups, and sexes in Baghdad for microscopic examination. One hundred randomly chosen patient and cattle stool samples underwent microscopic examination and conventional polymerase chain reaction (PCR) targeting the 18S rRNA gene. Phylogenetic tree analyses were performed for species identification.
The infection rate in humans differed significantly (p < 0.05) between age groups and genders, totaling 38%. The infection rate in cattle, determined by conventional PCR, differed significantly (p < 0.05) between age groups and genders, amounting to 58%. Ten PCR products with positive results were sequenced and deposited in GenBank database. Sequence analysis detected that Eight sequences belong to (OM268853.1, OM268854.1, OM268855.1, OM268857.1, OM268858.1, OM268860.1, OM268861.1, and OM268862.1) and two sequences belong to (OM268856.1 and OM268859.1) in humans, while 10 sequences (ON724165.1 to ON724174.1) belongs to in cattle.
The increased susceptibility of cattle to suggests a considerable role in human infection and substantial public health risks. Further research should be conducted on the many virulence factors such as HM1:IMSS strain, cysteinprotease (Cp1), Gal/lectin, etc. of and .
全球每年有10%的人口死于阿米巴病，记录在案的死亡人数达5万至10万。它与因人类粪便污染的食物和水源密切相关。该疾病的病理生理学仍是专家们持续争论的话题。一些专家认为宿主状况、寄生虫种类和菌株以及感染强度在引发临床症状方面起作用。本研究的目的是对从人和牛身上分离出的物种进行分子鉴定。
从巴格达不同地区、年龄组和性别的300名患者和100头牛采集粪便样本进行显微镜检查。对100份随机选取的患者和牛粪便样本进行显微镜检查以及针对18S rRNA基因的常规聚合酶链反应（PCR）。进行系统发育树分析以进行物种鉴定。
人类的感染率在年龄组和性别之间存在显著差异（p < 0.05），总计为38%。通过常规PCR确定的牛的感染率在年龄组和性别之间存在显著差异（p < 0.05），为58%。对10个PCR阳性产物进行测序并存入GenBank数据库。序列分析检测到人类中有8个序列属于（OM268853.1、OM268854.1、OM268855.1、OM268857.1、OM268858.1、OM268860.1、OM268861.1和OM268862.1），2个序列属于（OM268856.1和OM268859.1），而牛中有10个序列（ON724165.1至ON724174.1）属于。
牛对的易感性增加表明其在人类感染中起重要作用并带来重大公共卫生风险。应进一步研究和的许多毒力因子，如HM1:IMSS菌株、半胱氨酸蛋白酶（Cp1）、Gal/凝集素等。
