Wu Ze-Xuan, Xiao Han-Dan, He Yuan-Hui, Huang Shi-Bo, Li Jing, Kang Yu, Zheng Wen-Bin, Zhu Xing-Quan
Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China.
Vet Sci. 2025 Jan 4;12(1):19. doi: 10.3390/vetsci12010019.
spp. are common zoonotic intestinal protozoa, which can lead to serious intestinal diseases in both humans and animals through fecal-oral transmission, leading to significant economic losses and public health challenges. To reveal the prevalence of in sheep and cattle in Shanxi Province, North China, fecal samples were collected from 311 sheep, 392 dairy cattle, and 393 beef cattle from three representative counties in the northern, central, and southern regions of Shanxi Province. DNA was extracted from the fecal samples and amplified by PCR with primers targeting the nuclear small subunit ribosomal RNA (SSU rRNA) gene of spp., followed by the sequencing of the positive products. The overall infection rates of were 51.5% (160/311), 82.9% (325/392), and 79.1% (311/393) in sheep, dairy cattle, and beef cattle, respectively. Statistical analysis showed a significant correlation between the infection rate of and the location factor in sheep, dairy cattle, and beef cattle ( < 0.001). According to the obtained SSU rRNA sequences, several species, namely , Ribosomal Lineage (RL) 2, RL4, and RL8, were identified. This study represents the first molecular survey of prevalence in sheep, beef cattle, and dairy cattle in Shanxi Province. The findings extend the geographical distribution of spp. and provide valuable scientific data for the prevention and control of amoebiasis in Shanxi Province.
某些物种是常见的人畜共患肠道原生动物，可通过粪口传播导致人类和动物患上严重的肠道疾病，造成重大经济损失并带来公共卫生挑战。为揭示中国北方山西省绵羊和牛群中某些物种的流行情况，从山西省北部、中部和南部三个代表性县的311只绵羊、392头奶牛和393头肉牛采集了粪便样本。从粪便样本中提取DNA，并使用针对某些物种核小亚基核糖体RNA（SSU rRNA）基因的引物通过PCR进行扩增，随后对阳性产物进行测序。绵羊、奶牛和肉牛中某些物种的总体感染率分别为51.5%（160/311）、82.9%（325/392）和79.1%（311/393）。统计分析表明，绵羊、奶牛和肉牛中某些物种的感染率与地理位置因素之间存在显著相关性（P<0.001）。根据获得的SSU rRNA序列，鉴定出了几种某些物种，即某物种、核糖体谱系（RL）2、RL4和RL8。本研究是山西省绵羊、肉牛和奶牛中某些物种流行情况的首次分子调查。研究结果扩展了某些物种的地理分布范围，并为山西省阿米巴病的防控提供了有价值的科学数据。
