Microbiology Department, College of Medicine, Sulaimani University , Kurdistan Region , Iraq ; Basic Sciences Department, College of Medicine, Hawler Medical University , Kurdistan Region , Iraq.
Basic Sciences Department, College of Medicine, Hawler Medical University , Kurdistan Region , Iraq.
Pol J Microbiol. 2020;69(3):1-10. doi: 10.33073/pjm-2020-028.
The present study was conducted to evaluate the infection rates of , and among asymptomatic individuals in Erbil City, northern Iraq. The research intent was to discover whether pathogenic or nonpathogenic species cause a high rate of symptomless infections. Stool samples were microscopically examined, and the 18S-rRNA gene was targeted utilizing the nested PCR technique in the positive specimens. Initial results based on morphological features showed that the prevalence rate was 7.4%. Significantly higher rates of infections were seen in females than in males and in low-income people than in moderate-income people. The incidence rates among the asymptomatic individuals, as determined by molecular analysis, were as follows: - 6%, - 4.3%, and - 0.3%. Of all the positive samples, a single infection with was identified in 41.4% samples; the single infection with in 18.6% samples, 35.7% samples had mixed infections with two species, and 4.3% had mixed infections with three species. The current study concluded that 7.4% of healthy people, who live in the endemic area under investigation, carry species asymptomatically. Additionally, the majority of asymptomatic infections were caused by the pathogenic (81.4%) compared to (58.6%), and with the lowest rate of infection. Single and co-infections with and were noted. , which was identified for the first time in the region, was only seen in mixed infections. The present study was conducted to evaluate the infection rates of , and among asymptomatic individuals in Erbil City, northern Iraq. The research intent was to discover whether pathogenic or nonpathogenic species cause a high rate of symptomless infections. Stool samples were microscopically examined, and the 18S-rRNA gene was targeted utilizing the nested PCR technique in the positive specimens. Initial results based on morphological features showed that the prevalence rate was 7.4%. Significantly higher rates of infections were seen in females than in males and in low-income people than in moderate-income people. The incidence rates among the asymptomatic individuals, as determined by molecular analysis, were as follows: – 6%, – 4.3%, and – 0.3%. Of all the positive samples, a single infection with was identified in 41.4% samples; the single infection with in 18.6% samples, 35.7% samples had mixed infections with two species, and 4.3% had mixed infections with three species. The current study concluded that 7.4% of healthy people, who live in the endemic area under investigation, carry species asymptomatically. Additionally, the majority of asymptomatic infections were caused by the pathogenic (81.4%) compared to (58.6%), and with the lowest rate of infection. Single and co-infections with and were noted. , which was identified for the first time in the region, was only seen in mixed infections.
本研究旨在评估伊拉克北部埃尔比勒市无症状个体中 、 和 的感染率。研究目的是发现致病性或非致病性物种是否会导致无症状 感染率升高。对粪便样本进行显微镜检查，并在阳性样本中利用巢式 PCR 技术靶向 18S-rRNA 基因。基于形态特征的初步结果显示， 的流行率为 7.4%。女性的感染率明显高于男性，低收入人群的感染率明显高于中等收入人群。通过分子分析确定无症状个体的发病率如下： - 6%、- 4.3%和- 0.3%。在所有 阳性样本中，41.4%的样本中单一感染 ，18.6%的样本中单一感染 ，35.7%的样本中两种 物种混合感染，4.3%的样本中三种物种混合感染。本研究得出结论，生活在调查流行地区的 7.4%健康人无症状携带 物种。此外，与 相比，大多数无症状 感染是由致病性 （81.4%）引起的，而 感染率最低。注意到 与 的单感染和混合感染。在该地区首次发现的 仅见于混合感染。
